anti-Human, Rat E2F-1 Monoclonal Antibody | anti-E2F-1 antibody

Anti-E2F-1 Mouse mAb

Cat. Num. AAA475158

• Gene Names: E2F1; RBP3; E2F-1; RBAP1; RBBP3
• Reactivity: Human, Rat
• Applications: Western Blot, Immunofluorescence, Immunoprecipitation
• Purity: Affinity Purified
• Synonyms: E2F-1; Monoclonal Antibody; Anti-E2F-1 Mouse mAb; Dmel\CG6376; Dmel_CG6376; drosE2F1; E (Sev-CycE)3A; E (var)3-93E; E2-promoter binding facto; E2F 1; E2F transcription factor 1; E2f-PA; E2f-PB; E2f-PC; E2F1; E2f1 E2F transcription factor 1; E2F1_HUMAN; Evar (3)164; KIAA4009; l (3)07172; l (3)j3B1; l (3)j3C2; l (3)rM729; mKIAA4009; OTTHUMP00000030661; PBR3; PRB binding protein E2F 1; PRB-binding protein E2F-1; RBAP 1; RBAP-1; RBAP1; RBBP-3; RBBP3; RBP 3; RBP3; Retinoblastoma-associated protein 1; Retinoblastoma-binding protein 3; Transcription factor E2F1; anti-E2F-1 antibody

• Host: Mouse; Source: Human
• Reactivity: Human, Rat
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 4G8-2C2-C12
• Purity/Purification: Affinity Purified
• Form/Format: Purified mouse monoclonal antibody in PBS (pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
• Concentration: 5.79mg/mL (varies by lot)
• Sequence Length: 437

• Applicable Applications for anti-E2F-1 antibody: Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500; ICC: 1:100
• Immunogen: Purified recombinant human E2F-1 protein fragments expressed in E Coli.
• Sensitivity: This antibody detects endogenous levels of E2F-1 and does not cross-react with related proteins.
• Sequence Similarities: Belongs to the E2F/DP family.
• Subunit Structure: Component of the DRTF1/E2F transcription factor complex. Forms heterodimers with DP family members. The E2F1 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active. Viral oncoproteins, notably E1A, T-antigen and HPV E7, are capable of sequestering RB1, thus releasing the active complex. Interacts with TRRAP, which probably mediates its interaction with histone acetyltransferase complexes, leading to transcription activation. Binds TOPBP1 and EAPP. Interacts with ARID3A. Interacts with TRIM28; the interaction inhibits E2F1 acetylation through recruiting HDAC1 and represses its transcriptional activity. Interaction with KAT2B; the interaction acetylates E2F1 enhancing its DNA-binding and transcriptional activity. Interacts with BIRC2/c-IAP1 (via BIR domains). Interacts with human cytomegalovirus/HHV-5 protein UL123.
• Subcellular Location: Nucleus

Western Blot (WB)

(Western blot detection of E2F-1 in C6, Raw264.7 and Hela cell lysates using E2F-1 mouse mAb (1:500 diluted).Predicted band size:70KDa.Observed band size:70KDa.)

Immunofluorescence (IF)

(Immunofluorescent analysis of Hela cells fixed with 4% Paraformaldehyde and using anti-E2F-1 mouse mAb (dilution 1:100). DAPI was used to stain nucleus (blue).)

Immunoprecipitation (IP)

(Immunoprecipitation analysis of Hela cell lysates using E2F-1 mouse mAb.)

Related Product Information for anti-E2F-1 antibody

Entrez Summary: The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

Product Categories/Family for anti-E2F-1 antibody

Epigenetics and Nuclear Signaling; Transcription; Domain Families; Forkhead Box; Epigenetics and Nuclear Signaling; Transcription; Other factors; Cancer; Oncoproteins/suppressors; Tumor suppressors; Rb famil

NCBI and Uniprot Product Information

• NCBI GI #: 12669911
• NCBI GeneID: 1869
• NCBI Accession #: NP_005216.1
• NCBI GenBank Nucleotide #: NM_005225
• UniProt Accession #: Q01094; Q13143; Q92768
• Molecular Weight: 70
• NCBI Official Full Name: transcription factor E2F1
• NCBI Official Synonym Full Names: E2F transcription factor 1
• NCBI Official Symbol: E2F1
• NCBI Official Synonym Symbols: RBP3; E2F-1; RBAP1; RBBP3
• NCBI Protein Information: transcription factor E2F1
• UniProt Protein Name: Transcription factor E2F1
• UniProt Gene Name: E2F1
• UniProt Synonym Gene Names: RBBP3; E2F-1; RBAP-1; RBBP-3

NCBI Description

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq, Jul 2008]

Uniprot Description

E2F1: a member of the E2F/DP family of transcription factors.The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. Binds DNA cooperatively with Dp transcription factors in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication.The Dp-1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. The E2F-1 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active. Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase. It can mediate both cell proliferation and p53-dependent apoptosis.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 20q11.22

Cellular Component: nuclear chromatin; nucleoplasm; nucleus; Rb-E2F complex

Molecular Function: DNA binding; protein binding; sequence-specific DNA binding; transcription factor activity; transcription factor binding

Biological Process: DNA damage checkpoint; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA damage response, signal transduction resulting in induction of apoptosis; mRNA stabilization; negative regulation of DNA binding; negative regulation of fat cell differentiation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of fibroblast proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of transcription, DNA-dependent; transcription, DNA-dependent