Western Blot (WB)
(Western blot detection of E2F-1 in C6,Raw264.7 and Hela cell lysates using E2F-1 mouse mAb (1:500 diluted).Predicted band size:70KDa.Observed band size:70KDa.)
Mouse anti-Human E2F-1 Monoclonal Antibody | anti-E2F1 antibody
E2F-1 Antibody
ICC ~~ 1:100
IP ~~ 1:500
Western Blot (WB)
(Western blot detection of E2F-1 in C6,Raw264.7 and Hela cell lysates using E2F-1 mouse mAb (1:500 diluted).Predicted band size:70KDa.Observed band size:70KDa.)
Western Blot (WB)
(Western blot detection of E2F-1 in C6,Raw264.7 and Hela cell lysates using E2F-1 mouse mAb (1:500 diluted).Predicted band size:70KDa.Observed band size:70KDa.)
Immunocytochemistry (ICC)
(Immunofluorescent analysis of Hela cells fixed with 4% Paraformaldehyde and using anti-E2F-1 mouse mAb (dilution 1:100). DAPI was used to stain nucleus(blue).)
Immunocytochemistry (ICC)
(Immunofluorescent analysis of Hela cells fixed with 4% Paraformaldehyde and using anti-E2F-1 mouse mAb (dilution 1:100). DAPI was used to stain nucleus(blue).)
Immunoprecipitation (IP)
(Immunoprecipitation analysis of Hela cell lysates using E2F-1 mouse mAb.)
Immunoprecipitation (IP)
(Immunoprecipitation analysis of Hela cell lysates using E2F-1 mouse mAb.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq, Jul 2008]
Uniprot Description
E2F1: a member of the E2F/DP family of transcription factors.The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. Binds DNA cooperatively with Dp transcription factors in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication.The Dp-1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. The E2F-1 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active. Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase. It can mediate both cell proliferation and p53-dependent apoptosis.
Protein type: Transcription factor; DNA-binding
Chromosomal Location of Human Ortholog: 20q11.2
Cellular Component: nucleoplasm; Rb-E2F complex; cytoplasm; nuclear chromatin; nucleus
Molecular Function: protein binding; DNA binding; sequence-specific DNA binding; protein kinase binding; transcription factor activity; transcription factor binding
Biological Process: Notch signaling pathway; transcription, DNA-dependent; apoptosis; mRNA stabilization; positive regulation of transcription, DNA-dependent; programmed cell death; negative regulation of transcription from RNA polymerase II promoter; negative regulation of fat cell differentiation; negative regulation of DNA binding; anoikis; positive regulation of fibroblast proliferation; regulation of transcription, DNA-dependent; DNA damage response, signal transduction resulting in induction of apoptosis; forebrain development; DNA damage checkpoint; spermatogenesis; positive regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; negative regulation of transcription, DNA-dependent; G1/S transition of mitotic cell cycle
Research Articles on E2F1
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Product Notes
The E2F1 e2f1 (Catalog #AAA9215482) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The E2F-1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's E2F-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP). WB ~~ 1:500 ICC ~~ 1:100 IP ~~ 1:500. Researchers should empirically determine the suitability of the E2F1 e2f1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "E2F-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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