Mouse Dermatopontin (DPT) ELISA Kit | DPT elisa kit

Mouse Dermatopontin (DPT) ELISA Kit

Cat. Num. AAA450668

• Gene Names: Dpt; Eq1; EQ-1; 1810032B19Rik; 5033416F05Rik
• Reactivity: Mouse
• Synonyms: Dermatopontin (DPT); Mouse Dermatopontin (DPT) ELISA Kit; TRAMP; Tyrosine-rich acidic matrix protein; DPT elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of DPT. No significant cross-reactivity or interference between DPT and analogues was observed.
• Sequence Length: 201

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.057ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DPT were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DPT were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DPT elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of DPT in mouse tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DPT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to DPT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DPT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of DPT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 9789943
• NCBI GeneID: 56429
• NCBI Accession #: NP_062733.1
• NCBI GenBank Nucleotide #: NM_019759.2
• UniProt Accession #: Q9QZZ6; Q9D2B6
• Molecular Weight: 23,995 Da
• NCBI Official Full Name: dermatopontin
• NCBI Official Synonym Full Names: dermatopontin
• NCBI Official Symbol: Dpt
• NCBI Official Synonym Symbols: Eq1; EQ-1; 1810032B19Rik; 5033416F05Rik
• NCBI Protein Information: dermatopontin
• UniProt Protein Name: Dermatopontin
• Protein Family: Dermatopontin
• UniProt Gene Name: Dpt
• UniProt Synonym Gene Names: EQ-1; TRAMP

Uniprot Description

Seems to mediate adhesion by cell surface integrin binding. May serve as a communication link between the dermal fibroblast cell surface and its extracellular matrix environment. Enhances TGFB1 activity (). Inhibits cell proliferation. Accelerates collagen fibril formation, and stabilizes collagen fibrils against low-temperature dissociation.