Mouse anti-Human Dynamin-1-like Protein Monoclonal Antibody | anti-DNM1L antibody

Dynamin-1-like Protein (DNM1L, Dynamin Related Protein 1, DLP1, DRP1, Dynamin-like Protein, Dnm1p/Vps1p-like Protein, DVLP, DYMPLE, DYNIV-11, FLJ41912, HDYNIV, VPS1) (AP)

Cat. Num. AAA6131027

• Gene Names: DNM1L; DLP1; DRP1; DVLP; EMPF; EMPF1; DYMPLE; HDYNIV
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Dynamin-1-like Protein; Monoclonal Antibody; Dynamin-1-like Protein (DNM1L; Dynamin Related Protein 1; DLP1; DRP1; Dynamin-like Protein; Dnm1p/Vps1p-like Protein; DVLP; DYMPLE; DYNIV-11; FLJ41912; HDYNIV; VPS1) (AP); anti-DNM1L antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 3B5
• Specificity: Recognizes human DNM1L.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-DNM1L antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P: 1ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-710 from DNM1L (AAH24590) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MEALIPVINKLQDVFNTVGADIIQLPQIVVVGTQSSGKSSVLESLVGRDLLPRGTGIVTRRPLILQLVHVSQEDKRKTTGEENGVEAEEWGKFLHTKNKLYTDFDEIRQEIENETERISGNNKGVSPEPIHLKIFSPNVVNLTLVDLPGMTKVPVGDQPKDIELQIRELILRFISNPNSIILAVTAANTDMATSEALKISREVDPDGRRTLAVITKLDLMDAGTDAMDVLMGRVIPVKLGIIGVVNRSQLDINNKKSVTDSIRDEYAFLQKKYPSLANRNGTKYLARTLNRLLMHHIRDCLPELKTRINVLAAQYQSLLNSYGEPVDDKSATLLQLITKFATEYCNTIEGTAKYIETSELCGGARICYIFHETFGRTLESVDPLGGLNTIDILTAIRNATGPRPALFVPEVSFELLVKRQIKRLEEPSLRCVELVHEEMQRIIQHCSNYSTQELLRFPKLHDAIVEVVTCLLRKRLPVTNEMVHNLVAIELAYINTKHPDFADACGLMNNNIEEQRRNRLARELPSAVSRDKLIQDSRRETKNVASGGGGVGDGVQEPTTGNWRGMLKTSKAEELLAEEKSKPIPIMPASPQKGHAVNLLDVPVPVARKLSAREQRDCEVIERLIKSYFLIVRKNIQDSVPKAVMHFLVNHVKDTLQSELVGQLYKSSLLDDLLTESEDMAQRRKEAADMLKALQGASQIIAEIRETHLW
• Conjugate: AP
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (103.84kD).)

Western Blot (WB)

(DNM1L monoclonal antibody Western Blot analysis of DNM1L expression in K-562.)

Western Blot (WB)

(Western Blot analysis of DNM1L expression in transfected 293T cell line by DNM1L monoclonal antibody. Lane 1: DNM1L transfected lysate (79kD). Lane 2: Non-transfected lysate.)

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to DNM1L on formalin-fixed paraffin-embedded human leiomyosarcoma tissue. [antibody concentration 1ug/ml])

Testing Data

(Detection limit for recombinant GST tagged DNM1L is ~0.3ng/ml as a capture antibody.)

Product Categories/Family for anti-DNM1L antibody

Antibodies; Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 19352980
• NCBI GeneID: 10059
• Molecular Weight: 82,057 Da
• NCBI Official Full Name: Homo sapiens dynamin 1-like, mRNA
• NCBI Official Synonym Full Names: dynamin 1 like
• NCBI Official Symbol: DNM1L
• NCBI Official Synonym Symbols: DLP1; DRP1; DVLP; EMPF; EMPF1; DYMPLE; HDYNIV
• NCBI Protein Information: dynamin-1-like protein
• Protein Family: Dynamin-1-like protein

NCBI Description

This gene encodes a member of the dynamin superfamily of GTPases. The encoded protein mediates mitochondrial and peroxisomal division, and is involved in developmentally regulated apoptosis and programmed necrosis. Dysfunction of this gene is implicated in several neurological disorders, including Alzheimer's disease. Mutations in this gene are associated with the autosomal dominant disorder, encephalopathy, lethal, due to defective mitochondrial and peroxisomal fission (EMPF). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2013]