Mouse anti-Human DYKDDDDK Monoclonal Antibody
Mouse Anti DYKDDDDK peptide Clone PAT3G6AT
Reactivity
Human
Purity
Purified from mouse ascitic fluids by Protein-A affinity chromatography.
Synonyms
DYKDDDDK; Monoclonal Antibody; Mouse Anti DYKDDDDK peptide Clone PAT3G6AT; DYKDDDDK peptide Clone PAT3G6AT; Mouse Anti Human; DYKDDDDK PAT3G6AT; DYKDDDDK PAT3G6AT antibody; anti-DYKDDDDK antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1 heavy chain and k light chain.
Clone Number
PAT3G6AT.
Purity/Purification
Purified from mouse ascitic fluids by Protein-A affinity chromatography.
Form/Format
Sterile filtered colorless solution.
1mg/ml containing PBS, pH-7.4, 10% Glycerol and 0.02% Sodium Azide.
1mg/ml containing PBS, pH-7.4, 10% Glycerol and 0.02% Sodium Azide.
Application Notes
The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Immunogen
Anti-human DYKDDDDK mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a Synthetic peptide (DYKDDDDKC)-KLH.
Preparation and Storage
For 12 months store at -20 degree C. For 1 month store at 4 degree C.
Prevent freeze thaw cycles.
Prevent freeze thaw cycles.
Related Product Information for anti-DYKDDDDK antibody
A fusion tag called DYKDDDDK consists of eight amino acids (AspTyrLysAspAspAspAspLys) including an enterokinase-cleavage site. DYKDDDDK is specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions. Several antibodies against this peptide have been developed. One antibody denoted as M1 binds the peptide in the presence of bivalent metal cations preferably Ca(+). Elution is effected by chelating agents. Another strategy is competitive elution with excess of free DYKDDDDK Peptide. The DYKDDDDK peptide purifies and detects recombinant fusion proteins. DYKDDDDK peptide is useful in Western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization. DYKDDDDK peptide is a hydrophilic tag which significantly improves the detection and purification of recombinant fusion proteins.
Product Categories/Family for anti-DYKDDDDK antibody
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Product Notes
The DYKDDDDK (Catalog #AAA141207) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti DYKDDDDK peptide Clone PAT3G6AT reacts with Human and may cross-react with other species as described in the data sheet. The antibody has been tested by ELISA, Western blot analysis, Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Researchers should empirically determine the suitability of the DYKDDDDK for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DYKDDDDK, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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