Mouse Dopamine Receptor, D3 Monoclonal Antibody
Dopamine Receptor, D3
Reactivity
Human, Monkey, Rat
Applications
ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
Dopamine Receptor; D3; Monoclonal Antibody; Anti -Dopamine Receptor; anti-Dopamine Receptor; D3 antibody
Host
Mouse
Reactivity
Human, Monkey, Rat
Clonality
Monoclonal
Isotype
IgG3
Clone Number
3G137
Specificity
Recognizes human Dopamine Receptor, D3 at ~50kD. In immunoprecipitation experiments, additional bands are observed at higher molecular weights that appear to correspond to D3 multimers. Species Crossreactivity: Monkey and rat.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Applicable Applications for anti-Dopamine Receptor, D3 antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry.
Dilution: ELISA (Immunogen): 0.1-1ug/ml
Western Blot (Monkey, rat): 1-2ug/ml
Immunoprecipitation (native Human monkey, rat): 2-5ug
Immunohistochemistry (Human monkey, rat): 1-5ug/ml
Dilution: ELISA (Immunogen): 0.1-1ug/ml
Western Blot (Monkey, rat): 1-2ug/ml
Immunoprecipitation (native Human monkey, rat): 2-5ug
Immunohistochemistry (Human monkey, rat): 1-5ug/ml
Immunogen
Fusion protein incorporating aa252-284 of the putative third cytoplasmic loop of the human D3 receptor.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-Dopamine Receptor, D3 antibody
The 5 major receptors for the monoamine neurotransmitter dopamine are usually classified into two subfamilies based on biochemical, pharmacological and physiological properties. D1 subfamily receptors include the D1 and D5 (D1b) receptors. D2 subfamily receptors include the D2, D3, and D4 receptors. Each of these receptors exhibit the general structural characteristics of G-protein coupled receptors with seven transmembrane domains, an extracellular N-terminal domain, three extracellular loops, and four cytoplasmic loops, the last of which is formed by membrane insertion of a palmitoylated residue located in the intracellular C-terminal domain. The third intracellular loop is thought to be important for interaction with G-proteins. D1 subfamily receptors are characterized by short third intracellular loops and long C-terminal domains and generally stimulate adenylate cyclase production. D2 subfamily receptors have long third intracellular loops and short C-terminal domains and in general, appear to inhibit adenylate cyclase production. The D2 and D3 receptors vary in certain tissues and species as a result of alternative splicing.
Product Categories/Family for anti-Dopamine Receptor, D3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
D3 dopamine receptor
Similar Products
Product Notes
The Dopamine Receptor, D3 (Catalog #AAA609203) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Dopamine Receptor, D3 reacts with Human, Monkey, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Dopamine Receptor, D3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Dilution: ELISA (Immunogen): 0.1-1ug/ml Western Blot (Monkey, rat): 1-2ug/ml Immunoprecipitation (native Human monkey, rat): 2-5ug Immunohistochemistry (Human monkey, rat): 1-5ug/ml. Researchers should empirically determine the suitability of the Dopamine Receptor, D3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Dopamine Receptor, D3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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