Mouse DNMT1 Monoclonal Antibody | anti-DNMT1 antibody

DNMT1 Antibody: FITC

Cat. Num. AAA802232

• Gene Names: DNMT1; AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN
• Reactivity: Human, Mouse, Zebrafish
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Synonyms: DNMT1; Monoclonal Antibody; DNMT1 Antibody: FITC; DNA Mtase; DNMT; MCMT; DNA methyltransferase 1; AIM; CXXC9; DNA (cytosine-5)-methyltransferase 1; CXXC-type zinc finger protein 9; DNA methyltransferase HsaI; anti-DNMT1 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Zebrafish
• Clonality: Monoclonal
• Isotype: IgG1 Kappa
• Clone Number: 60B1220.1
• Form/Format: Protein G Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 1616

• Applicable Applications for anti-DNMT1 antibody: Western (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ChIp
• Application Notes: 2-4ug/mL (WB), 1-2ug/mL (IHC)
• Conjugate: FITC
• Storage Buffer: PBS, 0.05% BSA, 0.05% sodium azide
• Preparation and Storage: -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Mouse Anti-DNMT1 Monoclonal Antibody, Clone 60B1220.1. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-DNMT1 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Magnification: 40x. ABC solution 1:1000 dilution, DAB solution 100-200uL/ slide.)

Western Blot (WB)

(Western Blot analysis of Human H1299 cell lysate showing detection of DNMT1 protein using Mouse Anti-DNMT1 Monoclonal Antibody, Clone 60B1220.1. Primary Antibody: Mouse Anti-DNMT1 Monoclonal Antibody at 1:1000.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Mouse Anti-DNMT1 Monoclonal Antibody, Clone 60B1220.1. Tissue: medullar kidney tissue. Species: Mouse. Primary Antibody: Mouse Anti-DNMT1 Monoclonal Antibody at 1:1000. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown). Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain.)

Related Product Information for anti-DNMT1 antibody

Background Info: It will cross-react with mouse DNMT1.

Scientific Background: Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells (1-6).

Product Categories/Family for anti-DNMT1 antibody

Cell Signaling; Epigenetics; Organelle Markers

References

1. Bestor T., et al.(1988) J.Mol. Biol. 203: 971-983. 2. Yen R.W., Vertino P.M., Nelkin B.D. et al. (1992) Nucl. Acids Res. 20: 2287-2291. 3. Xie S. et al. (1999) Gene 236: 87-95. 4. Okano M., Bell D.W., Haber D.A. and Li E. (1999) Cell 99: 247-257. 5. Reik W. et al. (1999) J. Nat. Genet 23: 380-382. 6. Robertson K.D., et al. (2000) Nat Genet 25(3):338-342.

NCBI and Uniprot Product Information

• NCBI GI #: 4503351
• NCBI GeneID: 1786
• NCBI Accession #: NP_001370
• NCBI GenBank Nucleotide #: NM_001379.2
• UniProt Accession #: P26358; Q9UHG5; Q9ULA2; Q9UMZ6; A0AV63; B7ZLW6
• Molecular Weight: 144,465 Da
• NCBI Official Full Name: DNA (cytosine-5)-methyltransferase 1 isoform b
• NCBI Official Synonym Full Names: DNA (cytosine-5-)-methyltransferase 1
• NCBI Official Symbol: DNMT1
• NCBI Official Synonym Symbols: AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN
• NCBI Protein Information: DNA (cytosine-5)-methyltransferase 1; DNA (cytosine-5)-methyltransferase 1; m.HsaI; DNA MTase HsaI; DNA methyltransferase HsaI; CXXC-type zinc finger protein 9
• UniProt Protein Name: DNA (cytosine-5)-methyltransferase 1
• UniProt Gene Name: DNMT1
• UniProt Synonym Gene Names: AIM; CXXC9; DNMT; Dnmt1; DNA MTase HsaI; M.HsaI
• UniProt Entry Name: DNMT1_HUMAN

NCBI Description

DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

Uniprot Description

DNMT1: an ubiquitous DNA methyltransferase that methylates CpG residues. Preferentially methylates hemimethylated DNA. It is responsible for maintaining methylation patterns established in development, and may play an active role in DNA damage repair. Mediates transcriptional repression by direct binding to HDAC2. Its abundance is reduced to non detectable levels at the G0 phase of the cell cycle and is dramatically induced upon entrance into the S-phase of the cell cycle. Interacts with HDAC1 and with PCNA. Forms a complex with DMAP1 and HDAC2, with direct interaction. Forms also a stable complex with E2F1, BB1 and HDAC1. Binds MBD2 and MBD3. Three isoforms of the human protein produced by alternative splicing have been described.

Protein type: Methyltransferase; EC 2.1.1.37; Transcription regulation; Methyltransferase, DNA; Amino Acid Metabolism - cysteine and methionine; Cell development/differentiation

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: nucleoplasm; centric heterochromatin; replication fork; nucleus

Molecular Function: DNA (cytosine-5-)-methyltransferase activity; protein binding; methyl-CpG binding; DNA binding; zinc ion binding; RNA binding; chromatin binding; transcription factor binding; DNA-methyltransferase activity

Biological Process: negative regulation of histone H3-K9 methylation; transcription, DNA-dependent; maintenance of DNA methylation; negative regulation of transcription from RNA polymerase II promoter; chromatin modification; positive regulation of histone H3-K4 methylation; gene silencing; regulation of gene expression, epigenetic; regulation of cell proliferation; negative regulation of gene expression, epigenetic; DNA methylation; gene expression; DNA methylation on cytosine

Disease: Cerebellar Ataxia, Deafness, And Narcolepsy, Autosomal Dominant; Neuropathy, Hereditary Sensory, Type Ie