Mouse DNA Ligase 1 Monoclonal Antibody | anti-LIG1 antibody

DNA Ligase 1

Cat. Num. AAA4381062

• Reactivity: Human, Mouse, Rat, Bovine
• Applications: Western Blot, ELISA, Immunoprecipitation
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: DNA Ligase 1; Monoclonal Antibody; DNA ligase 1; DNA ligase I; DNLI1_HUMAN; LIG 1; lig1; Ligase I DNA ATP dependent; MGC117397; MGC130025; Polydeoxyribonucleotide synthase [ATP] 1; anti-LIG1 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat, Bovine
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: 1A9
• Specificity: DNA Ligase I maintains the major DNA Ligase activity in proliferating cells by joining Okazaki fragments during lagging strand DNA replication. Human DNA Ligase I also has an essential role in DNA repair pathways, where it catalyzes the formation of phosphodiester bonds between adjacent 5' phosphoryl and 3' hydroxy termini at single breaks in duplex DNA molecules. In addition, DNA Ligase I plays a role in sealing nicks during excision repair. Similar to other DNA ligases, DNA Ligase I is built around a common catalytic core. Increased levels of DNA Ligase I are found in human tumors, as compared to benign tissues, as well as in peripheral blood lymphocytes. Furthermore, DNA Ligase I antisense ODNs syndrome (BS). Individuals with BS either have decreased levels of abnormally thermolabile DNA Ligase I or possess a dimeric form of this enzyme.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 919

• Applicable Applications for anti-LIG1 antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: WB: 0.5-1ug/ml
• Positive Control: MOLT-4, PC-3 or Jurkat cell lysates.
• Immunogen: Full-length native cow LIG1 protein
• Cellular Localization: Nucleus.
• Hu-chromosome Location: 19q13.33
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

References

Barnes, D.E., et al. 1990. Human DNA Ligase I cDNA: cloning and functional expression in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 87: 6679-6683.

NCBI and Uniprot Product Information

• NCBI GI #: 118773
• NCBI GeneID: 3978
• UniProt Accession #: P18858
• Molecular Weight: 133kDa
• NCBI Official Full Name: DNA ligase 1
• NCBI Official Synonym Full Names: DNA ligase 1
• NCBI Official Symbol: LIG1
• NCBI Protein Information: DNA ligase 1
• UniProt Protein Name: DNA ligase 1
• Protein Family: DNA ligase
• UniProt Gene Name: LIG1
• UniProt Entry Name: DNLI1_HUMAN

NCBI Description

This gene encodes a member of the ATP-dependent DNA ligase protein family. The encoded protein functions in DNA replication, recombination, and the base excision repair process. Mutations in this gene that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. Disruption of this gene may also be associated with a variety of cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

LIG1: is an enzyme that functions in DNA replication and the base excision repair process. Mutations leading to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents.

Protein type: Ligase; EC 6.5.1.1; DNA repair, damage

Chromosomal Location of Human Ortholog: 19q13.2-q13.3

Cellular Component: nucleoplasm; Golgi apparatus; intracellular membrane-bound organelle; mitochondrion; chromosome; nucleus

Molecular Function: DNA binding; DNA ligase activity; metal ion binding; DNA ligase (ATP) activity; ATP binding

Biological Process: anatomical structure morphogenesis; mismatch repair; V(D)J recombination; DNA strand elongation during DNA replication; DNA repair; lagging strand elongation; double-strand break repair via homologous recombination; double-strand break repair via nonhomologous end joining; telomere maintenance via semi-conservative replication; response to hydrogen peroxide; base-excision repair; nucleotide-excision repair; cell division; transcription-coupled nucleotide-excision repair; double-strand break repair; telomere maintenance via recombination; DNA ligation during DNA repair; nucleotide-excision repair, DNA gap filling; mitotic cell cycle; telomere maintenance; DNA metabolic process