Mouse anti-Human DLL4 Monoclonal Antibody | anti-DLL4 antibody

Anti-DLL4 Antibody, Mouse Monoclonal

Cat. Num. AAA8101477

• Gene Names: DLL4; AOS6; delta4; hdelta2
• Reactivity: Human
• Applications: ELISA, ELISA
• Purity: Protein A
• Synonyms: DLL4; Monoclonal Antibody; Anti-DLL4 Antibody; Mouse Monoclonal; DLL4/Delta-4 Antibody; Mouse MAb; delta-like 4 (Drosophila); Anti-Delta-like 4 Antibody; Anti-hdelta2 Antibody; anti-DLL4 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 1C11D1G7
• Specificity: Human DLL4/Delta-4
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-DLL4 antibody: ELISA (EIA), ELISA (EIA) (Cap)
• Application Notes: ELISA: 1:1000-1:2000; ELISA (Cap): 1:250-1:2000; ELISA (Cap): This antibody will detect Human DLL4/Delta-like 4 in ELISA pair set.; In a sandwich ELISA, it can be used as capture antibody when paired with.
• Immunogen: Recombinant Human DLL4 protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human DLL4/Delta4 extracellular domain (rh DLL4). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-DLL4 antibody

Delta-like protein 4 (DLL4, Delta4), a type I membrane-bound Notch ligand, is one of five known Notch ligands in mammals and interacts predominantly with Notch 1, which has a key role in vascular development. Recent studies yield substantial insights into the role of DLL4 in angiogenesis. DLL4 is induced by vascular endothelial growth factor (VEGF) and acts downstream of VEGF as a 'brake' on VEGF-induced vessel growth, forming an autoregulatory negative feedback loop inactivating VEGF. DLL4 is downstream of VEGF signaling and its activation triggers a negative feedback that restrains the effects of VEGF. Attenuation of DLL4/Notch signaling results in chaotic vascular network with excessive branching and sprouting. DLL4 is widely distributed in tissues other than vessels including many malignancies. Furthermore, the molecule is internalized on binding its receptor and often transported to the nucleus. In pathological conditions, such as cancer, DLL4 is up-regulated strongly in the tumour vasculature. Blockade of DLL4-mediated Notch signaling strikingly increases nonproductive angiogenesis, but significantly inhibits tumor growth in preclinical mouse models. In preclinical studies, blocking of DLL4/Notch signaling is associated with a paradoxical increase in tumor vessel density, yet causes marked growth inhibition due to functionally defective vasculature. Thus, DLL4 blockade holds promise as an additional strategy for angiogenesis-based cancer therapy.

Product Categories/Family for anti-DLL4 antibody

Cancer Drug Targets Notch Pathway

References

Yan M, et al. (2007) Delta-like 4/Notch signaling and its therapeutic implications. Clin Cancer Res. 13(24): 7243-6.Sainson RC, et al. (2007) Anti-Dll4 therapy: can we block tumour growth by increasing angiogenesis? Trends Mol Med. 13(9): 389-95.Martinez JC, et al. (2009) Nuclear and membrane expression of the angiogenesis regulator delta-like ligand 4 (DLL4) in normal and malignant human tissues. Histopathology. 54(5): 598-606.Li JL, et al. (2010) Targeting DLL4 in tumors shows preclinical activity but potentially significant toxicity. Future Oncol. 6(7): 1099-103.

NCBI and Uniprot Product Information

• NCBI GI #: 9506545
• NCBI GeneID: 54567
• NCBI Accession #: NP_061947.1
• NCBI GenBank Nucleotide #: NM_019074.4
• UniProt Accession #: Q9NR61; Q3KP23; Q9NQT9
• Molecular Weight: 74,605 Da
• NCBI Official Full Name: delta-like protein 4
• NCBI Official Synonym Full Names: delta like canonical Notch ligand 4
• NCBI Official Symbol: DLL4
• NCBI Official Synonym Symbols: AOS6; delta4; hdelta2
• NCBI Protein Information: delta-like protein 4
• UniProt Protein Name: Delta-like protein 4
• Protein Family: Delta-like protein
• UniProt Gene Name: DLL4
• UniProt Synonym Gene Names: Delta4

NCBI Description

This gene is a homolog of the Drosophila delta gene. The delta gene family encodes Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. [provided by RefSeq, Jul 2008]

Uniprot Description

Involved in the Notch signaling pathway as Notch ligand (PubMed:11134954). Activates NOTCH1 and NOTCH4. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting (PubMed:20616313). Essential for retinal progenitor proliferation. Required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types (). During spinal cord neurogenesis, inhibits V2a interneuron fate (PubMed:17728344).