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Western Blot (WB) (Figure 1: Western blot analysis using DLK1 mAb against human DLK1 (AA: 174-349) recombinant protein. (Expected MW is 44.9 kDa))

Mouse anti-Human DLK1 Monoclonal Antibody | anti-DLK1 antibody

DLK1 Antibody

Gene Names
DLK1; DLK; FA1; ZOG; pG2; DLK-1; PREF1; Delta1; Pref-1
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA, Flow Cytometry, Functional Assay
Purity
Affinity-Chromatography
Synonyms
DLK1; Monoclonal Antibody; DLK1 Antibody; Adipocyte differentiation inhibitor protein; Brevideltin; Delta like 1 homolog; Delta like homolog; Delta like protein; Delta1; DLK 1; DLK; DLK-1; DLK1_HUMAN; FA 1; FA1; Fetal antigen 1; pG2; Preadipocyte factor 1; Pref 1; Pref; Pref1; Protein delta homolog 1; Secredeltin; ZOG; anti-DLK1 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Specificity
DLK1 antibody detects endogenous levels of total DLK1
Purity/Purification
Affinity-Chromatography
Form/Format
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
310
Applicable Applications for anti-DLK1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA), Flow Cytometry (FC/FACS)
Application Notes
ELISA: 1:10000
WB: 1:500-1:2000
ICC: 1:200-1:1000
FC/FACS: 1:200-1:400
Immunogen
Purified recombinant fragment of human DLK1 expressed in E. Coli
Subcellular Location
Membrane.
Tissue Specificity
Found within the stromal cells in close contact to the vascular structure of placental villi, yolk sac, fetal liver, adrenal cortex and pancreas and in the beta cells of the islets of Langerhans in the adult pancreas. Found also in some forms of neuroendocrine lung tumor tissue.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Figure 1: Western blot analysis using DLK1 mAb against human DLK1 (AA: 174-349) recombinant protein. (Expected MW is 44.9 kDa))

Western Blot (WB) (Figure 1: Western blot analysis using DLK1 mAb against human DLK1 (AA: 174-349) recombinant protein. (Expected MW is 44.9 kDa))
Related Product Information for anti-DLK1 antibody
Description: This gene encodes a transmembrane protein containing six epidermal growth factor repeats. The protein is involved in the differentiation of several cell types, including adipocytes; it is also thought to be a tumor suppressor. It is one of several imprinted genes located in a region of on chr 14q32. Certain mutations in this imprinted region can cause phenotypes similar to maternal and paternal uniparental disomy of chromosome 14 (UPD14). This gene is expressed from the paternal allele. A polymorphism within this gene has been associated with child and adolescent obesity. The mode of inheritance for this polymorphism is polar overdominance; this non-Mendelian inheritance pattern was first described in sheep with the callipyge phenotype, which is characterized by muscle hypertrophy and decreased fat mass.
Function: May have a role in neuroendocrine differentiation.
Subunit Structure: Monomer.
Post-translational Modifications: N-and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 41 kDa
Predicted: 42 kDa
NCBI Official Full Name
protein delta homolog 1 isoform 2 preproprotein
NCBI Official Synonym Full Names
delta like non-canonical Notch ligand 1
NCBI Official Symbol
DLK1
NCBI Official Synonym Symbols
DLK; FA1; ZOG; pG2; DLK-1; PREF1; Delta1; Pref-1
NCBI Protein Information
protein delta homolog 1
UniProt Protein Name
Protein delta homolog 1
Protein Family
UniProt Gene Name
DLK1
UniProt Synonym Gene Names
DLK; DLK-1; FA1

NCBI Description

This gene encodes a transmembrane protein that contains multiple epidermal growth factor repeats that functions as a regulator of cell growth. The encoded protein is involved in the differentiation of several cell types including adipocytes. This gene is located in a region of chromosome 14 frequently showing unparental disomy, and is imprinted and expressed from the paternal allele. A single nucleotide variant in this gene is associated with child and adolescent obesity and shows polar overdominance, where heterozygotes carrying an active paternal allele express the phenotype, while mutant homozygotes are normal. [provided by RefSeq, Nov 2015]

Uniprot Description

May have a role in neuroendocrine differentiation.

Research Articles on DLK1

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Product Notes

The DLK1 dlk1 (Catalog #AAA9602638) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The DLK1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DLK1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA), Flow Cytometry (FC/FACS). ELISA: 1:10000 WB: 1:500-1:2000 ICC: 1:200-1:1000 FC/FACS: 1:200-1:400. Researchers should empirically determine the suitability of the DLK1 dlk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DLK1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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