Rabbit anti-Human, Mouse ILF3/NF90 Monoclonal Antibody | anti-ILF3 antibody

ILF3/NF90 Rabbit mAb

Cat. Num. AAA4752828

• Gene Names: ILF3; CBTF; DRBF; MMP4; MPP4; NF90; NFAR; NF110; NF90a; NF90b; NFAR2; TCP80; DRBP76; NF110b; NFAR-1; TCP110; MPHOSPH4; NF-AT-90
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, Immunoprecipitation
• Purity: Affinity purified
• Synonyms: ILF3/NF90; Monoclonal Antibody; ILF3/NF90 Rabbit mAb; CBTF; DRBF; MMP4; MPP4; NF90; NFAR; NF110; NF90a; NF90b; NFAR2; TCP80; DRBP76; NF110b; NFAR-1; TCP110; MPHOSPH4; NF-AT-90; anti-ILF3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Specificity: Ubiquitous. Gene expression databases
• Purity/Purification: Affinity purified
• Form/Format: 50nM Tris-Glycine (pH7.4), 0.15M Nacl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

• Applicable Applications for anti-ILF3 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
• Application Notes: WB: 1:2000-1:20000; IHC: 1:50; ICC/IF: 1:100; FC/FACS: 1:20; IP: 1:20
• Immunogen: A synthetic peptide of human ILF3
• Conjugation: Unconjugated
• Modification: Unmodification
• Subcellular Location: Cytoplasm, Nucleus
• Preparation and Storage: Store at 4 degree C short term. Store at -20 degree C long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western blot detection of ILF3/NF90 in Jurkat,C6,Hela cell lysates using ILF3/NF90 Rabbit mAb(1:1000 diluted). Predicted band size:95kDa. Observed band size:95kDa.)

Related Product Information for anti-ILF3 antibody

This gene encodes a double-stranded RNA (dsRNA) binding protein that complexes with other proteins, dsRNAs, small noncoding RNAs, and mRNAs to regulate gene expression and stabilize mRNAs. This protein (NF90, ILF3) forms a heterodimer with a 45kDa transcription factor (NF45, ILF2) required for T-cell expression of interleukin 2. This complex has been shown to affect the redistribution of nuclear mRNA to the cytoplasm. Knockdown of NF45 or NF90 protein retards cell growth, possibly by inhibition of mRNA stabilization. In contrast, an isoform (NF110) of this gene that is predominantly restricted to the nucleus has only minor effects on cell growth when its levels are reduced. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Dec 2014]
RNA-binding protein that plays an essential role in the biogenesis of circular RNAs (circRNAs) which are produced by back-splicing circularization of pre-mRNAs. Within the nucleus, promotes circRNAs processing by stabilizing the regulatory elements residing in the flanking introns of the circularized exons. Plays thereby a role in the back-splicing of a subset of circRNAs (PubMed:28625552). As a consequence, participates in a wide range of transcriptional and post-transcriptional processes. Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs (PubMed:14731398). Upon viral infection, ILF3 accumulates in the cytoplasm and participates in the innate antiviral response (PubMed:21123651). Mechanistically, ILF3 becomes phosphorylated and activated by the double-stranded RNA-activated protein kinase/PKR which releases ILF3 from cellular mature circRNAs. In turn, unbound ILF3 molecules are able to interact with and thus inhibit viral mRNAs (PubMed:21123651, PubMed:28625552).

NCBI and Uniprot Product Information

• NCBI GI #: 62512150
• NCBI GeneID: 3609
• UniProt Accession #: Q12906; O43409; Q6P1X1; Q86XY7; Q99544; Q99545; Q9BZH4; Q9BZH5; Q9NQ95; Q9NQ96; A8K6F2; G5E9M5
• Molecular Weight: 894
• NCBI Official Full Name: Interleukin enhancer-binding factor 3
• NCBI Official Synonym Full Names: interleukin enhancer binding factor 3, 90kDa
• NCBI Official Symbol: ILF3
• NCBI Official Synonym Symbols: CBTF; DRBF; MMP4; MPP4; NF90; NFAR; NF110; NF90a; NF90b; NFAR2; TCP80; DRBP76; NF110b; NFAR-1; TCP110; MPHOSPH4; NF-AT-90
• NCBI Protein Information: interleukin enhancer-binding factor 3; M-phase phosphoprotein 4; translational control protein 80; dsRNA binding protein NFAR-2/MPP4; nuclear factor associated with dsRNA; double-stranded RNA-binding protein, 76 kD; nuclear factor of activated T-cells 90
• UniProt Protein Name: Interleukin enhancer-binding factor 3
• Protein Family: Interleukin enhancer-binding factor
• UniProt Gene Name: ILF3
• UniProt Synonym Gene Names: DRBF; MPHOSPH4; NF90; DRBP76; MPP4; NFAR; NF-AT-90; TCP80
• UniProt Entry Name: ILF3_HUMAN

NCBI Description

This gene encodes a double-stranded RNA (dsRNA) binding protein that complexes with other proteins, dsRNAs, small noncoding RNAs, and mRNAs to regulate gene expression and stabilize mRNAs. This protein (NF90, ILF3) forms a heterodimer with a 45 kDa transcription factor (NF45, ILF2) required for T-cell expression of interleukin 2. This complex has been shown to affect the redistribution of nuclear mRNA to the cytoplasm. Knockdown of NF45 or NF90 protein retards cell growth, possibly by inhibition of mRNA stabilization. In contrast, an isoform (NF110) of this gene that is predominantly restricted to the nucleus has only minor effects on cell growth when its levels are reduced. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Dec 2014]

Uniprot Description

NFAT90: a double-stranded RNA (dsRNA)-binding protein implicated in the regulation of gene expression . Phosphorylated in a double-stranded RNA-dependent manner possibly by PKR. Five different splice-variant isoforms have been described.

Protein type: RNA-binding; Transcription factor; DNA-binding; Nucleolus

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: nucleoplasm; membrane; mitochondrion; cytoplasm; nucleolus; ribonucleoprotein complex; nucleus

Molecular Function: protein binding; DNA binding; RNA binding; double-stranded RNA binding

Biological Process: transcription, DNA-dependent; negative regulation of viral genome replication; positive regulation of transcription, DNA-dependent; negative regulation of translation; negative regulation of transcription, DNA-dependent; defense response to virus; protein amino acid phosphorylation