anti-Human DGKa Monoclonal Antibody | anti-dgkA antibody
Mouse anti DGKa
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Synonyms
DGKa; Monoclonal Antibody; Mouse anti DGKa; anti-dgkA antibody
Host
Mouse (Balb/c)
Reactivity
Human
Clonality
Monoclonal
Clone Number
M8
Specificity
M8 is directed anti the C-terminal portion (part of the Catalytic domain) of Rat DGKa.
Form/Format
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
Applicable Applications for anti-dgkA antibody
Immunoprecipitation (IP), Western Blot (WB)
Application Notes
M8 is useful for immunoprecipitation and western blot. Optimal antibody dilution should be determined by titRation.
Source Note
M8 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with an Escherichia coli cell-expressed, affinity-purified glutathione S-transferase fusion protein of a C-terminal portion (part of the Catalytic domain) of Rat DGKu.
Preparation and Storage
Store at 4 degree C, or in small aliquots at -20 degree C.
Related Product Information for anti-dgkA antibody
Diacylglycerol kinase alpha is an enzyme that in Humans is encoded by the DGKA gene. The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. Tissue specificity: lymphocytes and oligodendroglial cells. Diacylglycerol kinases are involved in cell signaling, either as regulators of diacylglycerol levels or as intracellular signal-geneRating enzymes.
References
Brahim Houssa, Dick Schaap, Jose´ van der Wal, Kaoru Gotoi, Hisatake Kondoi, Akio Yamakawa, Masao Shibata, Tadaomi Takenawa, and Wim J. van Blitterswijk THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 272, No. 16, Issue of April 18, pp. 10422-10428, 1997. Santina Cutrupi. Src-mediated activation of -diacylglycerol kinase is required for hepatocyte growth factor-induced cell motility. EMBO.2000, vol 19,46-13.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
DgkA
NCBI Official Symbol
dgkA
NCBI Protein Information
diacylglycerol kinase DgkA; hypothetical protein
Protein Family
Uniprot Description
Recycling of diacylglycerol produced during the turnover of membrane phospholipid.
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Product Notes
The dgkA (Catalog #AAA570271) is an Antibody produced from Mouse (Balb/c) and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti DGKa reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DGKa can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Western Blot (WB). M8 is useful for immunoprecipitation and western blot. Optimal antibody dilution should be determined by titRation. Researchers should empirically determine the suitability of the dgkA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DGKa, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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