Mouse Desmoglein-2 (DSG2) Monoclonal Antibody | anti-DSG2 antibody

Desmoglein-2 (DSG2)

Cat. Num. AAA439915

• Gene Names: DSG2; HDGC; CDHF5
• Reactivity: Human. Others not known.
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
• Synonyms: Desmoglein-2 (DSG2); Monoclonal Antibody; ARVC10; ARVD10; Cadherin family member 5; CDHF5; CMD1BB; Desmoglein-2; DSG2; HDGC; Human Desmoglein Colon; anti-DSG2 antibody

• Host: Mouse
• Reactivity: Human. Others not known.
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: 6D8
• Specificity: Recognizes a protein of 165kDa, identified as Desmoglein-2 (DSG2). This monoclonal antibody recognizes the extracellular domain of human desmoglein-2. Desmoglein-2 is a member of the desmosomal cadherin family. Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. Desmocollins and desmogleins are the main desmosomal transmembrane proteins. Desmogleins consist of Dsg1, Dsg2, Dsg3, and Dsg4 isoforms. Within the desmosome, the extracellular domain of desmoglein is essential for calcium dependent heterophilic binding to the desmocollins, whereas the intracellular domain is essential for binding to the desmosomal plaque protein, plakoglobin. Human Desmoglein-2 is a type I transmembrane glycoprotein of 1117 amino acid (aa) residues with a 23 aa signal peptide and a 25 aa propeptide. It differs from other classic cadherins by having four instead of five cadherin repeat domains in its extracellular region, and a much larger cytoplasmic region containing five desmoglein repeat domains which share homology with the cadherin repeats. Instead of having the HAV adhesion motif found in type I cadherins, desmogleins have R/YAL as the adhesion motif on its amino-terminal cadherin repeat. The cytoplasmic tails of desmogleins interact with desmoplakins, plakoglobin and plakophilins. In turn, these proteins link the desmogleins with the intermediate filaments. Desmoglein-2 has been shown to be important in establishing cell-cell adhesion and function in epithelial cells. Desmoglein2 was originally identified in colon carcinoma and colon, and was named HDGC (human desmoglein colon).
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 1118

• Applicable Applications for anti-DSG2 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: FC/FACS: 0.5-1ug/million cells; IF: 1-2ug/ml; WB: 0.5-1.0ug/ml
• Immunogen: Recombinant human full-length DSG2 protein
• Cellular Localization: Cell Surface
• Hu-chromosome Location: 18q12.1
• Positive Control: A431, HepG2 or K562 cells. Heart or Colon
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C.; Antibody without azide - store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

References

Wahl, JK III, Nieset, JE; Sacco-Bubulya, PA; Sadler, TM; Johnson, KR; Wheelock, MJ. The amino- and carboxyl-terminal tails of (beta)-catenin reduce its affinity for desmoglein 2. J Cell Sci. (2000), 113 :1737-45. Arnemann, J; Spurr, NK; Magee, AI; Buxton RS. The human gene (DSG2) coding for HDGC, a second member of the desmoglein subfamily of the desmosomal cadherins, is, like DSG1 coding for desmoglein DGI, assigned to chromosome 18. Genomics (1992), 13(2), 484-.

NCBI and Uniprot Product Information

• NCBI GI #: 116534898
• NCBI GeneID: 1829
• NCBI Accession #: NP_001934.2
• NCBI GenBank Nucleotide #: NM_001943.4
• UniProt Accession #: Q14126; Q4KKU6
• Molecular Weight: 165kDa
• NCBI Official Full Name: desmoglein-2 preproprotein
• NCBI Official Synonym Full Names: desmoglein 2
• NCBI Official Symbol: DSG2
• NCBI Official Synonym Symbols: HDGC; CDHF5
• NCBI Protein Information: desmoglein-2
• UniProt Protein Name: Desmoglein-2
• Protein Family: Desmoglein
• UniProt Gene Name: DSG2
• UniProt Synonym Gene Names: CDHF5

NCBI Description

This gene encodes a member of the desmoglein family and cadherin cell adhesion molecule superfamily of proteins. Desmogleins are calcium-binding transmembrane glycoprotein components of desmosomes, cell-cell junctions between epithelial, myocardial, and other cell types. The encoded preproprotein is proteolytically processed to generate the mature glycoprotein. This gene is present in a gene cluster with other desmoglein gene family members on chromosome 18. Mutations in this gene have been associated with arrhythmogenic right ventricular dysplasia, familial, 10. [provided by RefSeq, Jan 2016]

Uniprot Description

Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.