Mouse anti-Human DEP1 Monoclonal Antibody | anti-DEP1 antibody

DEP1 (PTPRJ, Receptor-type Tyrosine-protein Phosphatase eta, Protein-tyrosine Phosphatase eta, R-PTP-eta, Density-enhanced Phosphatase 1, DEP-1, HPTP eta, Protein-tyrosine Phosphatase Receptor Type J, R-PTP-J, CD148) (PE)

Cat. Num. AAA6157424

• Gene Names: PTPRJ; DEP1; SCC1; CD148; HPTPeta; R-PTP-ETA
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DEP1; Monoclonal Antibody; DEP1 (PTPRJ; Receptor-type Tyrosine-protein Phosphatase eta; Protein-tyrosine Phosphatase eta; R-PTP-eta; Density-enhanced Phosphatase 1; DEP-1; HPTP eta; Protein-tyrosine Phosphatase Receptor Type J; R-PTP-J; CD148) (PE); anti-DEP1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 6G11
• Specificity: Recognizes human PTPRJ.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 1337

• Applicable Applications for anti-DEP1 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Partial recombinant corresponding to aa38-138, from human PTPRJ (NP_002834) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: GTPSPIPDPSVATVATGENGITQISSTAESFHKQNGTGTPQVETNTSEDGESSGANDSLRTPEQGSNGTDGASQKTPSSTGPSPVFDIKAVSISPTNVIL
• Conjugate: PE
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Testing Data

(Detection limit for recombinant GST tagged PTPRJ is ~0.03ng/ml as a capture antibody.)

Related Product Information for anti-DEP1 antibody

DEP1 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region containing five fibronectin type III repeats, a single transmembrane region, and a single intracytoplasmic catalytic domain, and thus represents a receptor-type PTP. This PTP is present in all hematopoietic lineages, and was shown to negatively regulate T cell receptor signaling possibly through interfering with the phosphorylation of Phospholipase C Gamma 1 (PLCG1) and Linker for Activation of T Cells (LAT). This PTP was also found to dephosphorylate PDGF beta receptor, and may be involved in UV-induced signal transduction.

Product Categories/Family for anti-DEP1 antibody

Antibodies; Enzymes; Phosphatase

NCBI and Uniprot Product Information

• NCBI GI #: 148728162
• NCBI GeneID: 5795
• NCBI Accession #: NP_002834
• NCBI GenBank Nucleotide #: NM_002843
• UniProt Accession #: Q12913
• NCBI Official Full Name: receptor-type tyrosine-protein phosphatase eta isoform 1
• NCBI Official Synonym Full Names: protein tyrosine phosphatase receptor type J
• NCBI Official Symbol: PTPRJ
• NCBI Official Synonym Symbols: DEP1; SCC1; CD148; HPTPeta; R-PTP-ETA
• NCBI Protein Information: receptor-type tyrosine-protein phosphatase eta
• UniProt Protein Name: Receptor-type tyrosine-protein phosphatase eta
• Protein Family: Transcriptional regulatory protein
• UniProt Gene Name: PTPRJ
• UniProt Synonym Gene Names: DEP1; Protein-tyrosine phosphatase eta; R-PTP-eta; DEP-1; R-PTP-J

NCBI Description

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes, including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region containing five fibronectin type III repeats, a single transmembrane region, and a single intracytoplasmic catalytic domain, and thus represents a receptor-type PTP. This protein is present in all hematopoietic lineages, and was shown to negatively regulate T cell receptor signaling possibly through interfering with the phosphorylation of Phospholipase C Gamma 1 and Linker for Activation of T Cells. This protein can also dephosphorylate the PDGF beta receptor, and may be involved in UV-induced signal transduction. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Tyrosine phosphatase which dephosphorylates or contributes to the dephosphorylation of CTNND1, FLT3, PDGFRB, MET, RET (variant MEN2A), KDR, LYN, SRC, MAPK1, MAPK3, EGFR, TJP1, OCLN, PIK3R1 and PIK3R2. Plays a role in cell adhesion, migration, proliferation and differentiation. Involved in vascular development. Regulator of macrophage adhesion and spreading. Positively affects cell-matrix adhesion. Positive regulator of platelet activation and thrombosis. Negative regulator of cell proliferation. Negative regulator of PDGF-stimulated cell migration; through dephosphorylation of PDGFR. Positive regulator of endothelial cell survival, as well as of VEGF-induced SRC and AKT activation; through KDR dephosphorylation. Negative regulator of EGFR signaling pathway; through EGFR dephosphorylation. Enhances the barrier function of epithelial junctions during reassembly. Negatively regulates T-cell receptor (TCR) signaling. Upon T-cell TCR activation, it is up-regulated and excluded from the immunological synapses, while upon T-cell-antigen presenting cells (APC) disengagement, it is no longer excluded and can dephosphorylate PLCG1 and LAT to down-regulate prolongation of signaling.