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Testing Data

Mouse anti-Rat Dendritic cell Monoclonal Antibody

Anti-Rat Dendritic cell Purified (Clone OX-62) (mouse IgG1)

Reactivity
Rat
Applications
Flow Cytometry
Synonyms
Dendritic cell; Monoclonal Antibody; Anti-Rat Dendritic cell Purified (Clone OX-62) (mouse IgG1); Dendritic cell Purified (Clone OX-62) (mouse IgG1); Anti-Rat Dendritic Cell (CD103) Monoclonal Antibody; anti-Dendritic cell antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Rat
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
OX-62
Specificity
CD103 (Dendritic Cells) (alpha E2 Intergrin) (Rat lymphoid and non lymphoid dendritic cells)
Form/Format
Purified
Concentration
1.0 mg/ml (varies by lot)
Applicable Applications for anti-Dendritic cell antibody
Flow cytometry (FC)
Application Notes
Flow cytometry: This antibody clone OX-62 has also been reported to work in IP, cell surface radioiodination and in immunohistochemistry with cryostat sections. The clone does not appear to work well with paraffin sections.

Recommended Isotype Control:
Mouse IgG1 Isotype control: MBS524513
Hybridoma Production
Spleen cells from BALB/c mice immunized with rat PVG density gradient-enriched veiled cells (obtained from the cannulated thoracic duct of mesenteric lymphadenectomized rats) were fused with NSO myeloma cells.
Presentation
Purified IgG buffered in PBS and 0.02% NaN3. (Purified from ascitic fluid via Protein G Chromatography).
Preparation and Storage
For all formats, store at 4 degree C. For long term storage (Ascites, Purified, FITC), aliquot and freeze unused portion at -20 degree C in volumes appropriate for single usage. Avoid freeze/thaw cycles.

Testing Data

Testing Data
Related Product Information for anti-Dendritic cell antibody
OX-62 monoclonal antibody (generally referred to as CD103) was raised against density gradientenriched rat veiled (dendritic) cells obtained from lymph. The antigen recognized is expressed at low to moderate levels by a subset of spleen, thymus and bone marrow dendritic cells. The antigen has the biochemical characteristics of an integrin as determined by SDS-PAGE. This monoclonal antibody is useful for cell purification, delineating lineage relationships among cells with dendritic morphology and for molecular studies. The clone has been use in flow cytometry, immunohistochemistry, immunoprecipitation and cell surface radioiodination.

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Product Notes

The Dendritic cell (Catalog #AAA520274) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Rat Dendritic cell Purified (Clone OX-62) (mouse IgG1) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Dendritic cell can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC). Flow cytometry: This antibody clone OX-62 has also been reported to work in IP, cell surface radioiodination and in immunohistochemistry with cryostat sections. The clone does not appear to work well with paraffin sections. Recommended Isotype Control: Mouse IgG1 Isotype control: MBS524513. Researchers should empirically determine the suitability of the Dendritic cell for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Dendritic cell, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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