Human Neurogranin (NRGN) ELISA Kit | NRGN elisa kit

Human Neurogranin (NRGN) ELISA Kit

Cat. Num. AAA2700107

• Gene Names: NRGN; RC3; hng
• Reactivity: Human
• Synonyms: Neurogranin (NRGN); Human Neurogranin (NRGN) ELISA Kit; Ng; NEUG; Protein Kinase C Substrate RC3; NRGN elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Neurogranin (NRGN).; No significant cross-reactivity or interference between Neurogranin (NRGN) and analogues was observed.
• Sequence Length: 78

• Assay Type: Competitive Inhibition
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 39-10000pg/mL
• Sensitivity: < 17pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neurogranin (NRGN) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neurogranin (NRGN) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NRGN elisa kit

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Neurogranin (NRGN) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Neurogranin (NRGN) and unlabeled Neurogranin (NRGN) (Standards or samples) with the pre-coated antibody specific to Neurogranin (NRGN). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Neurogranin (NRGN) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Neurogranin (NRGN) in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 5453800
• NCBI GeneID: 4900
• NCBI Accession #: NP_006167.1
• NCBI GenBank Nucleotide #: NM_006176.2
• UniProt Accession #: Q92686
• Molecular Weight: 7,618 Da
• NCBI Official Full Name: neurogranin
• NCBI Official Synonym Full Names: neurogranin
• NCBI Official Symbol: NRGN
• NCBI Official Synonym Symbols: RC3; hng
• NCBI Protein Information: neurogranin
• UniProt Protein Name: Neurogranin
• Protein Family: Neurogranin
• UniProt Gene Name: NRGN
• UniProt Synonym Gene Names: Ng

NCBI Description

Neurogranin (NRGN) is the human homolog of the neuron-specific rat RC3/neurogranin gene. This gene encodes a postsynaptic protein kinase substrate that binds calmodulin in the absence of calcium. The NRGN gene contains four exons and three introns. The exons 1 and 2 encode the protein and exons 3 and 4 contain untranslated sequences. It is suggested that the NRGN is a direct target for thyroid hormone in human brain, and that control of expression of this gene could underlay many of the consequences of hypothyroidism on mental states during development as well as in adult subjects. [provided by RefSeq, Jul 2008]

Uniprot Description

Acts as a "third messenger" substrate of protein kinase C-mediated molecular cascades during synaptic development and remodeling. Binds to calmodulin in the absence of calcium ().