Mouse anti-Human DAXX Monoclonal Antibody | anti-DAXX antibody

DAXX (Transcriptional Corepressor) Mouse Monoclonal Antibody [Clone PCRP-DAXX-5G11]

Cat. Num. AAA4381820

• Gene Names: DAXX; DAP6; EAP1; BING2
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
• Synonyms: DAXX; Monoclonal Antibody; DAXX (Transcriptional Corepressor) Mouse Monoclonal Antibody [Clone PCRP-DAXX-5G11]; DAXX (Transcriptional Corepressor); DAP6; EAP1; BING2; SMIM40; anti-DAXX antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse/IgG2b
• Clone Number: PCRP-DAXX-5G11
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0. 05% BSA & 0. 05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Concentration: Purified Ab with BSA and Azide at 200ug/ml; OR; Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)

• Applicable Applications for anti-DAXX antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry-Formalin (IHC-F)
• Application Notes: FC/FACS: 1-2ug/million cells; IF: 1-2ug/ml; IHC (Formalin-fixed): 1-2ug/ml for 30 minutes at RT; (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH9.0, for 45 minutes at 95 degree C followed by cooling at RT for 20 minutes)
• Immunogen: Recombinant full-length human DAXX protein
• Cellular Localization: Nucleus, Cytoplasm
• Positive Control: HeLa or U87 cells. Ubiquitous tissue expression.
• Hu-chromosome Location: 6p21.32
• Unigene: 336916
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C; Antibody without azide: store at -20 to -80 degree C; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-Page

(SDS-PAGE Analysis Purified DAXX Mouse Monoclonal Antibody (PCRP-DAXX-5G11). Confirmation of Purity and Integrity of Antibody. )

Related Product Information for anti-DAXX antibody

This gene encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants.

NCBI and Uniprot Product Information

• NCBI GI #: 215422388
• NCBI GeneID: 1616
• NCBI Accession #: NP_001135441.1
• NCBI GenBank Nucleotide #: NM_001141969.1
• UniProt Accession #: Q9UER7; O14747; O15141; O15208; Q5STK9; Q9BWI3; B4E1I3; F5H082
• Molecular Weight: 81,373 Da
• NCBI Official Full Name: death domain-associated protein 6 isoform a
• NCBI Official Synonym Full Names: death-domain associated protein
• NCBI Official Symbol: DAXX
• NCBI Official Synonym Symbols: DAP6; EAP1; BING2
• NCBI Protein Information: death domain-associated protein 6; Fas-binding protein; CENP-C binding protein; ETS1-associated protein 1; death-associated protein 6; fas death domain-associated protein
• UniProt Protein Name: Death domain-associated protein 6
• Protein Family: Death domain-associated protein
• UniProt Gene Name: DAXX
• UniProt Synonym Gene Names: BING2; DAP6; hDaxx; EAP1
• UniProt Entry Name: DAXX_HUMAN

NCBI Description

This gene encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2008]

Uniprot Description

DAXX: a transcriptional co-regulatory protein. Proposed to mediate activation of the JNK pathway and apoptosis via ASK1 in response to signaling from FAS and TGF-betaR2. Glucose deprivation activates the ASK1-SEK1-JNK1-HIPK1 pathway, relocalizing Daxx from the nucleus to the cytoplasm, where Daxx binds to ASK1, and subsequently leads to ASK1 oligomerization. Interaction with HSP27 may prevent interaction with TGF-betaR2 and ASK1 and block DAXX-mediated apoptosis. Seems to act as a transcriptional co- repressor and inhibits PAX3 and ETS1 through direct protein- protein interaction. Its transcription repressor activity is modulated by recruiting it to subnuclear compartments like the nucleolus or PML/POD/ND10 nuclear bodies through interactions with MCSR1 and PML, respectively. Two alternatively spliced isoforms have been described.

Protein type: Apoptosis; Transcription, coactivator/corepressor; Nucleolus; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: heterochromatin; PML body; cytoplasm; nucleolus; nucleus; cytosol; chromosome, pericentric region

Molecular Function: protein homodimerization activity; histone binding; p53 binding; protein N-terminus binding; protein kinase binding; transcription factor binding; protein binding; enzyme binding; androgen receptor binding; heat shock protein binding; ubiquitin protein ligase binding; receptor signaling protein activity; protein kinase activator activity; transcription corepressor activity

Biological Process: viral reproduction; transcription, DNA-dependent; regulation of protein ubiquitination; apoptosis; activation of JNK activity; chromatin remodeling; nucleosome assembly; cytokinesis after mitosis; regulation of transcription, DNA-dependent; induction of apoptosis via death domain receptors; positive regulation of protein kinase activity; androgen receptor signaling pathway; positive regulation of protein amino acid phosphorylation; negative regulation of transcription, DNA-dependent