Mouse anti-Human D4-GDI Monoclonal Antibody | anti-ARHGDIB antibody
D4-GDI (GDP Dissociation Inhibitor, Rho GDP-dissociation Inhibitor 2, Rho GDI 2, Ly-GDI, Rho-GDI beta, ARHGDIB, GDIA2, GDID4, RAP1GN1)
Reactivity
Human
Applications
Western Blot
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
D4-GDI; Monoclonal Antibody; D4-GDI (GDP Dissociation Inhibitor; Rho GDP-dissociation Inhibitor 2; Rho GDI 2; Ly-GDI; Rho-GDI beta; ARHGDIB; GDIA2; GDID4; RAP1GN1); Anti -D4-GDI (GDP Dissociation Inhibitor; anti-ARHGDIB antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Clone Number
4H73
Specificity
Recognizes human D4-GDI
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS 0.02% sodium azide, 0.02% gelatin.
Applicable Applications for anti-ARHGDIB antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 1-2ug/ml
Whole cell lysate from Daudi or Jurkat cell can be used as positive control and a band of approx. size of 28kD is detected.
Dilution: Western Blot: 1-2ug/ml
Whole cell lysate from Daudi or Jurkat cell can be used as positive control and a band of approx. size of 28kD is detected.
Immunogen
Synthetic peptide corresponding to human D4-GDI.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-ARHGDIB antibody
D4-GDI (GDP dissociation inhibitor) is a negative regulator of the ras-related Rho family of GTPases. Since the Rho GTPases promote cytoskeletal and membrane changes associated with apoptotic cell death, the removal of the D4-GDI block through its cleavage is important for inducing apoptosis. Caspase-3 cleaves the 28 kD mature form of D4-GDI to give a 23 kD and 5 kD size fragment. The 23 kD fragment then translocates to the nucleus. The mechanisms involving cleavage of D4-GDI with apoptosis are not presently known. Activation of the Jun N-terminal kinase, a regulator of apoptosis, may be one of the mechanisms.
Product Categories/Family for anti-ARHGDIB antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
22,794 Da
NCBI Official Full Name
D4-GDP-dissociation inhibitor
NCBI Official Symbol
ARHGDIB
NCBI Protein Information
rho GDP-dissociation inhibitor 2; D4-GDI; ly-GDI; rho GDI 2; rho-GDI beta; D4-GDP-dissociation inhibitor
UniProt Protein Name
Rho GDP-dissociation inhibitor 2
Protein Family
UniProt Gene Name
ARHGDIB
UniProt Synonym Gene Names
GDID4
UniProt Entry Name
GDIR2_BOVIN
Uniprot Description
Function: Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them.
Subcellular location: Cytoplasm.
Sequence similarities: Belongs to the Rho GDI family.
Similar Products
Product Notes
The ARHGDIB arhgdib (Catalog #AAA603456) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The D4-GDI (GDP Dissociation Inhibitor, Rho GDP-dissociation Inhibitor 2, Rho GDI 2, Ly-GDI, Rho-GDI beta, ARHGDIB, GDIA2, GDID4, RAP1GN1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's D4-GDI can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1-2ug/ml Whole cell lysate from Daudi or Jurkat cell can be used as positive control and a band of approx. size of 28kD is detected. Researchers should empirically determine the suitability of the ARHGDIB arhgdib for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "D4-GDI, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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