Mouse anti-Human Cytokeratin Type II Monoclonal Antibody | anti-KRT75 antibody

Mouse Monoclonal [clone AE3] (IgG1) to Human Cytokeratin Type II

Cat. Num. AAA246262

• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified IgG
• Synonyms: Cytokeratin Type II; Monoclonal Antibody; Mouse Monoclonal [clone AE3] (IgG1) to Human Cytokeratin Type II; Anti-Cytokeratin Type II Antibody (clone AE3) IHC-plus; Human Cytokeratin Type II; anti-KRT75 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: AE3
• Specificity: This antibody has shown to recognize most of the basic (type II) keratins. Each epithelium contains at least one basic keratin. In this way AE-3 is broadly reactive with almost all epithelial and there neoplasms. This antibody together with AE-1 has been used for studying the immuno-localization of epidermal keratins, tissue distribution and epithelia development. AE-1 and AE-3 have also been used extensively, individually or mixed, for distinguishing carcinoma from non-carcinoma. The antibody is cross-reactive with keratins of rabbit, mouse, rat, bovine and chicken. Within immunoblots AE-1 detects distinct MW's of 52 kD, 54 kD, 56 kD, 58 kD, 59 kD, 64 kD, and 65-67 kD with all Ip's lower then 6.0. These MW's are believed to correspond with respectively keratin 8, 7, 6, 5, 4, 3 and 1 & 2.
• Purity/Purification: Purified IgG
• Form/Format: 0.01M PBS containing 0.1% Sodium azide and 1% BSA. Sourced in Ascites.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 551

• Applicable Applications for anti-KRT75 antibody: Immunohistochemistry (IHC - Paraffin; IHC - Frozen), Western Blot (WB)
• Application Notes: IHC-Fr, IHC-P (1:100), WB (1:100); Usage: In unfixed frozen sections and formalin-fixed paraffin embedded tissues. Also applicable in Western blotting. For use on formalin-fixed paraffin embedded tissues a dilution of approximately 1:150 to 1:200 is appropriate if section are incubated for 3...
• Target Species: Human
• Immunogen Description: Human epidermal cells.
• Immunogen Type: Cells
• Immunogen: Human epidermal cells.
• Preparation and Storage: Store at 4 degree C.

Immunohistochemistry (IHC)

(Anti-Cytokeratin Type II antibody IHC of human intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)

Immunohistochemistry (IHC)

(Anti-Cytokeratin Type II antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)

NCBI and Uniprot Product Information

• NCBI GI #: 3901030
• UniProt Accession #: O95678; Q9NSA9; B4DQU4
• Molecular Weight: 59,560 Da
• NCBI Official Full Name: cytokeratin type II
• UniProt Protein Name: Keratin, type II cytoskeletal 75
• Protein Family: Keratin
• UniProt Gene Name: KRT75
• UniProt Synonym Gene Names: K6HF; KB18; CK-75; hK6hf; K75
• UniProt Entry Name: K2C75_HUMAN

Uniprot Description

KRT75: Plays a central role in hair and nail formation. Essential component of keratin intermediate filaments in the companion layer of the hair follicle. Defects in KRT75 may be a cause of loose anagen hair syndrome (LAHS). In LAHS, anagen hairs are easily pulled from the scalp. The hair is relatively sparse and does not grow long. Hair of fair color and hair shafts of reduced caliber, and an early age of onset are features. Usually the hairs are not fragile and there are no areas of breakage. Belongs to the intermediate filament family.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: keratin filament

Molecular Function: structural molecule activity

Biological Process: hemopoietic progenitor cell differentiation

Disease: Pseudofolliculitis Barbae