Mouse anti-Human Cytokeratin 8,18 Monoclonal Antibody | anti-KRT8 antibody

Cytokeratin 8,18

Cat. Num. AAA604998

• Gene Names: KRT8; K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Ascites; Ascites
• Synonyms: Cytokeratin 8; 18; Monoclonal Antibody; Anti -Cytokeratin 8; anti-KRT8 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 5F272
• Specificity: Species Crossreactivity: Human, No significant reaction with mouse or rat. Recognizes all simple epithelia including glandular epithelia. It does not react with stratified squamous epithelium. Useful for the differentiation between carcinomas and lymphomas. This clone is directed against the 52.2kD cytokeratin 8 (originally reported also to 45kD cytokeratin 18) in immunoblot. Epitope has been mapped to aa353-367 on alpha helical rod domain of cytokeratin 8.
• Purity/Purification: Ascites; Ascites
• Form/Format: Supplied as a liquid.

• Applicable Applications for anti-KRT8 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Immunoblot and Immunohistochemistry (frozen and paraffin-embedded tissue, Proteolytic treatment with pepsin or pronase).; Dilution: Immunohistochemistry: 1:5-1:10, dilute immediately before use.
• Immunogen: Isolated from human breast cancer carcinoma cell line MCF-7.
• Positive Control: Glandular epithelium
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degree C or colder. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Categories/Family for anti-KRT8 antibody

Antibodies; Abs to Cytokeratin

NCBI and Uniprot Product Information

• NCBI GI #: 30313
• NCBI GeneID: 3856
• UniProt Accession #: P05787; Q14099; Q14716; Q14717; Q53GJ0; Q6DHW5; Q6GMY0; Q6P4C7; Q96J60; A8K4H3; B0AZN5; F8VXB4
• Molecular Weight: 53,704 Da
• NCBI Official Full Name: cytokeratin 8 (279 AA)
• NCBI Official Synonym Full Names: keratin 8
• NCBI Official Symbol: KRT8
• NCBI Official Synonym Symbols: K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
• NCBI Protein Information: keratin, type II cytoskeletal 8; cytokeratin 8; cytokeratin-8; type-II keratin Kb8
• UniProt Protein Name: Keratin, type II cytoskeletal 8
• Protein Family: Keratin
• UniProt Gene Name: KRT8
• UniProt Synonym Gene Names: CYK8; CK-8; K8
• UniProt Entry Name: K2C8_HUMAN

NCBI Description

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

K8: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Phosphorylation of keratins at specific sites affects their organization, assembly dynamics, and their interaction with signaling molecules. Phsophorylated by p38 kinase, regulating cellular keratin filament reorganization. Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Mutation of this protein is a risk factor for cryptogenic liver failure.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: nucleoplasm; dystrophin-associated glycoprotein complex; intermediate filament cytoskeleton; nuclear matrix; costamere; keratin filament; cytoplasm; intermediate filament; intercellular junction; nucleus; Z disc; sarcolemma

Molecular Function: protein binding; protein complex binding; structural molecule activity

Biological Process: viral reproduction; tumor necrosis factor-mediated signaling pathway; response to other organism; sarcomere organization; response to hydrostatic pressure

Disease: Cirrhosis, Familial