Mouse anti-Human Cytokeratin 8/18 Monoclonal Antibody | anti-CK8/CK18 antibody

Cytokeratin 8/18

Cat. Num. AAA4381493

• Gene Names: KRT8; K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
• Purity: Culture Supernatant
• Synonyms: Cytokeratin 8/18; Monoclonal Antibody; CARD2; CK8; CYK8; CYKER; Cytokeratin Endo A; DreK8; EndoA; K2C8; K8; Keratin 8; Krt 2.8; KRT8; Type-II Keratin Kb8. Cell Proliferation-inducing Gene 46 Protein; CK18; CYK18Cytokeratin Endo B; K18; Keratin-18; Kerd; KRT18; anti-CK8/CK18 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: 5D3
• Specificity: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.
• Purity/Purification: Culture Supernatant
• Form/Format: Tissue culture supernatant with 0.05% Azide. Contact us if you require it in a different format.
• Sequence Length: 483

• Applicable Applications for anti-CK8/CK18 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin
• Application Notes: FC/FACS: 2-5ul/million cells; IF: 1:100-1:200; Immunohistochemistry (Formalin-fixed) (1:50-1:100 for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Positive Control: MCF-7 or A431 cells. Skin, colon, lung or breast carcinoma.
• Immunogen: Cytoskeleton preparation from human breast cancer MCF-7 cells
• Cellular Localization: Cytoplasmic
• Hu-chromosome Location: 17q21.2 (CK8); 12q13.13 (CK18)
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 8/18 Monoclonal Antibody (5D3).)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cytokeratin 8/18 Monoclonal Antibody (5D3).)

References

1. Bartkova et al. Neoplasma 38(4), 439-46 (1991). 2. Bartek et al. J. Pathology,164:215-24 (1991).

NCBI and Uniprot Product Information

• NCBI GI #: 372466577
• NCBI GeneID: 3856
• NCBI Accession #: NP_001243222.1
• NCBI GenBank Nucleotide #: NP_001243222.1
• UniProt Accession #: P05787; P05783
• Molecular Weight: 52.5kDa (CK8); 45kDa (CK18)
• NCBI Official Full Name: keratin, type II cytoskeletal 8 isoform 2
• NCBI Official Synonym Full Names: keratin 8
• NCBI Official Symbol: KRT8
• NCBI Official Synonym Symbols: K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
• NCBI Protein Information: keratin, type II cytoskeletal 8
• UniProt Protein Name: Keratin, type II cytoskeletal 8
• UniProt Gene Name: KRT8
• UniProt Synonym Gene Names: CYK8; CK-8; K8
• UniProt Entry Name: K2C8_HUMAN

NCBI Description

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

K8: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Phosphorylation of keratins at specific sites affects their organization, assembly dynamics, and their interaction with signaling molecules. Phsophorylated by p38 kinase, regulating cellular keratin filament reorganization. Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Mutation of this protein is a risk factor for cryptogenic liver failure.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: nucleoplasm; dystrophin-associated glycoprotein complex; intermediate filament cytoskeleton; costamere; nuclear matrix; keratin filament; cytoplasm; intermediate filament; intercellular junction; Z disc; nucleus; sarcolemma

Molecular Function: protein binding; protein complex binding; structural molecule activity

Biological Process: tumor necrosis factor-mediated signaling pathway; viral reproduction; sarcomere organization; response to other organism; response to hydrostatic pressure

Disease: Cirrhosis, Familial