Mouse anti-Human Cytokeratin 7,17 Monoclonal Antibody | anti-Cyb5 antibody

Cytokeratin 7,17

Cat. Num. AAA607496

• Gene Names: Cyb5; Cyb5a; 0610009N12Rik
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Cytokeratin 7; 17; Monoclonal Antibody; Anti -Cytokeratin 7; anti-Cyb5 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 10B2654
• Specificity: Recognizes a unique epitope shared by the intermediate filament keratins, of human cytokeratin 7 and cytokeratin 17, a 54kDa type II basic cytokeratin, and 46kD type I acidic cytokeratin respectively. Species Crossreactivity: bovine, porcine. Does not react with mouse, rabbit, rat.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

• Applicable Applications for anti-Cyb5 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Immunoprecipitation, Immunohistochemistry (paraffin), and Western Blot.; Dilution: Immunohistochemistry (paraffin): 20-30ug/ml; Western Blot: 2-3ug/ml; Requires protein digestion pre-treatment of paraffin sections using pepsin prior to staining.
• Immunogen: HeLa cell cytoskeleton preparation.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Categories/Family for anti-Cyb5 antibody

Antibodies; Abs to Cytokeratin

NCBI and Uniprot Product Information

• NCBI GI #: 13385268
• NCBI GeneID: 109672
• NCBI Accession #: NP_080073.1
• NCBI GenBank Nucleotide #: NM_025797.3
• UniProt Accession #: P56395
• Molecular Weight: 15,241 Da
• NCBI Official Full Name: cytochrome b5
• NCBI Official Synonym Full Names: cytochrome b-5
• NCBI Official Symbol: Cyb5
• NCBI Official Synonym Symbols: Cyb5a; 0610009N12Rik
• NCBI Protein Information: cytochrome b5
• UniProt Protein Name: Cytochrome b5
• UniProt Gene Name: Cyb5a
• UniProt Synonym Gene Names: Cyb5
• UniProt Entry Name: CYB5_MOUSE

Uniprot Description

CYB5A: Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. Defects in CYB5A are the cause of methemoglobinemia CYB5A-related (METHB-CYB5A). A form of methemoglobinemia, a hematologic disease characterized by the presence of excessive amounts of methemoglobin in blood cells, resulting in decreased oxygen carrying capacity of the blood, cyanosis and hypoxia. Belongs to the cytochrome b5 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: endoplasmic reticulum membrane; mitochondrion; membrane; intracellular membrane-bound organelle; endoplasmic reticulum; integral to membrane

Molecular Function: enzyme binding; metal ion binding; heme binding; stearoyl-CoA 9-desaturase activity

Biological Process: transport; fatty acid metabolic process