Mouse Cytokeratin 5,8 Monoclonal Antibody | anti-Krt8 antibody

Cytokeratin 5,8

Cat. Num. AAA604520

• Gene Names: Krt8; CYKER; Krt2-8
• Reactivity: Canine, Hamster, Human, Mammal, Mouse, Porcine, Rabbit, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Supernatant; Culture supernatant
• Synonyms: Cytokeratin 5; 8; Monoclonal Antibody; Anti -Cytokeratin 5; anti-Krt8 antibody

• Host: Mouse
• Reactivity: Canine, Hamster, Human, Mammal, Mouse, Porcine, Rabbit, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 5F295
• Specificity: Directed against the 58kD and 52.2kD cytokeratin proteins (cytokeratins 5 and 8), reacts with all epithelial cell types. No reaction is observed in non-epithelial tissues. Can be used for the discrimination of carcinomas from non-epithelial tumors such as lymphomas, sarcomas and neural tumors. Species Crossreactivity: Human, most mammals including mouse, rat, hamster, rabbit, canine and porcine.
• Purity/Purification: Supernatant; Culture supernatant
• Form/Format: Supplied as a liquid.

• Applicable Applications for anti-Krt8 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence, Immunoblot and Immunohistochemistry (frozen sections; paraffin sections, Proteolytic treatment with pronase or trypsin required; Formalin fixation may cause antigen denaturation or masking. Therefore, protease digestion is necessary prior to antibody application).; Dilution: Immunohistochemistry: 1:5-1:10
• Immunogen: Lung cancer cell preparation.
• Positive Control: Skin, cervix.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degree C or colder. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Categories/Family for anti-Krt8 antibody

Antibodies; Abs to Cytokeratin

NCBI and Uniprot Product Information

• NCBI GI #: 203734
• NCBI GeneID: 25626
• UniProt Accession #: Q10758; Q5WPB3; Q6LCB1
• Molecular Weight: 54,019 Da
• NCBI Official Full Name: cytokeratin 8 polypeptide
• NCBI Official Synonym Full Names: keratin 8
• NCBI Official Symbol: Krt8
• NCBI Official Synonym Symbols: CYKER; Krt2-8
• NCBI Protein Information: keratin, type II cytoskeletal 8; K8; CK-8; keratin-8; cytokeratin-8; cytokeratin endo A; type-II keratin Kb8; keratin complex 2, basic, gene 8
• UniProt Protein Name: Keratin, type II cytoskeletal 8
• Protein Family: Keratin
• UniProt Gene Name: Krt8
• UniProt Synonym Gene Names: Krt2-8
• UniProt Entry Name: K2C8_RAT

NCBI Description

an intermediate filament protein; may play a role in prostrate cells [RGD]

Uniprot Description

K8: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Phosphorylation of keratins at specific sites affects their organization, assembly dynamics, and their interaction with signaling molecules. Phsophorylated by p38 kinase, regulating cellular keratin filament reorganization. Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Mutation of this protein is a risk factor for cryptogenic liver failure.

Protein type: Cytoskeletal

Cellular Component: nucleoplasm; dystrophin-associated glycoprotein complex; intermediate filament cytoskeleton; costamere; nuclear matrix; keratin filament; cytoplasm; intermediate filament; intercellular junction; nucleus; Z disc; sarcolemma

Molecular Function: protein binding; protein complex binding; structural molecule activity

Biological Process: tumor necrosis factor-mediated signaling pathway; sarcomere organization; response to other organism; response to hydrostatic pressure