Cytokeratin 19 Monoclonal Antibody
Mouse Anti-Cytokeratin 19
Applications
Immunofluorescence, Flow Cytometry, Functional Assay, ELISA, Western Blot, Immunohistochemistry
Purity
Purified by Protein A/G affinity chromatography.
Synonyms
Cytokeratin 19; Monoclonal Antibody; Mouse Anti-Cytokeratin 19; k19; k1cs; Keratin 19 Keratin Type i 40kD; krt19 BSA & Azide Free; anti-Cytokeratin 19 antibody
Host
Host: Mouse; Source: Human
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
BA17
Specificity
Recognizes human Cytokeratin 19. Species Crossreactivity: mouse
Purity/Purification
Purified by Protein A/G affinity chromatography.
Form/Format
Supplied as a liquid in 10mM PBS. Also available with BSA and azide, see 168748.
Applicable Applications for anti-Cytokeratin 19 antibody
Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Flow Cytometry: 1-2ug/million cells Immunofluorescence: 1-2ug/ml. Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes Optimal dilutions to be determined by the researcher.
Crossreactivity
Human, Mouse
Immunogen
Human mammary epithelial organoids.
Cellular Localization
Cytoplasmic
Notes
Preservative Free, BSA Free
Positive Control
MCF-7, HeLa cells, human lung lysate, colon or breast cancer
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-Cytokeratin 19 antibody
This Ab reacts with the rod domain of human cytokeratin 19 (CK19), a polypeptide of 40kDa. CK19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.
References
1. Morton JP et. al. Am J Pathol 172:1081-7 (2008).
2. Olofsson MH et. al. Clin Cancer Res 13:3198-206 (2007).
3. Aleksic T et. al. Gut 56:227-36 (2007).
4. Nightingale J et. al. J Am Soc Nephrol 15:21-32 (2004).
5. Lewis BC et. al. Genes Dev 17:3127-38 (2003).
6. Bartek J et. al. J Cell Sci 75:17-33 (1985)
7. Bartek J et. al. Int J Cancer 36:299-306 (1985).
2. Olofsson MH et. al. Clin Cancer Res 13:3198-206 (2007).
3. Aleksic T et. al. Gut 56:227-36 (2007).
4. Nightingale J et. al. J Am Soc Nephrol 15:21-32 (2004).
5. Lewis BC et. al. Genes Dev 17:3127-38 (2003).
6. Bartek J et. al. J Cell Sci 75:17-33 (1985)
7. Bartek J et. al. Int J Cancer 36:299-306 (1985).
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Product Notes
The Cytokeratin 19 (Catalog #AAA6506770) is an Antibody produced from Host: Mouse; Source: Human and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Cytokeratin 19 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Flow Cytometry: 1-2ug/million cells Immunofluorescence: 1-2ug/ml. Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the Cytokeratin 19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytokeratin 19, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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