Mouse anti-Human Cytokeratin 16 (KRT16) Monoclonal Antibody | anti-KRT16 antibody

Cytokeratin 16 (KRT16) (Suprabasal Keratinocyte Marker)

Cat. Num. AAA4381059

• Gene Names: KRT16; K16; PC1; CK16; K1CP; NEPPK; FNEPPK; KRT16A
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Cytokeratin 16 (KRT16); Monoclonal Antibody; Cytokeratin 16 (KRT16) (Suprabasal Keratinocyte Marker); CK16; Cytokeratin-16; Focal non epidermolytic palmoplantar keratoderma (FNEPPK/NEPPK); K1CP; Keratin Type I Cytoskeletal 16; Keratin-16; KRT16A; anti-KRT16 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: SPM264
• Specificity: Cytokeratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I Cytokeratins (Cytokeratins 9 to 23) and one type II Cytokeratins (keratins 1 to 8). The cytokeratin proteins play a critical role in differentiation, as well as tissue specialization and function, to maintain the overall structural integrity of epithelial cells. Cytokeratins are also useful markers in identifying the origin of metastatic tumors. Cytokeratin 16 is expressed in benign stratified squamous epithelium and squamous cell carcinoma of the head and neck, as well as luminal cells of mammary gland and sweat ducts. It is absent in non-invasive breast carcinomas and normal breast tissue.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 473

• Applicable Applications for anti-KRT16 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Frozen
• Application Notes: FC/FACS: 0.5-1ug/million cells; IF: 1-2ug/ml; WB: 0.5-1ug/ml; Immunohistochemistry (Frozen & formalin-fixed) (0.5-1ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Positive Control: HeLa cells. Tonsil, Skin. Bladder or Cervix.
• Immunogen: Recombinant fragment from the C-terminal of human Cytokeratin 16
• Cellular Localization: Cytoplasmic
• Hu-chromosome Location: 17q21.2
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Tonsil stained with Cytokeratin 16 Mouse Monoclonal Antibody (SPM264).)

References

1. Porter et al. 2000. Lab Invest. 80(11):1701-10. 2. Wetzels et al. 1991. Am J Pathol. 138(3):751-63. 3. Markey et al. 1992. Br J Dermatol. 126(2):154-60.

NCBI and Uniprot Product Information

• NCBI GI #: 24430192
• NCBI GeneID: 3868
• NCBI Accession #: NP_005548.2
• NCBI GenBank Nucleotide #: NP_005548.2
• UniProt Accession #: P08779
• Molecular Weight: 48kDa
• NCBI Official Full Name: keratin, type I cytoskeletal 16
• NCBI Official Synonym Full Names: keratin 16
• NCBI Official Symbol: KRT16
• NCBI Official Synonym Symbols: K16; PC1; CK16; K1CP; NEPPK; FNEPPK; KRT16A
• NCBI Protein Information: keratin, type I cytoskeletal 16
• UniProt Protein Name: Keratin, type I cytoskeletal 16
• Protein Family: Keratin
• UniProt Gene Name: KRT16
• UniProt Synonym Gene Names: KRT16A; CK-16; K16
• UniProt Entry Name: K1C16_HUMAN

NCBI Description

The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region of chromosome 17q12-q21. This keratin has been coexpressed with keratin 14 in a number of epithelial tissues, including esophagus, tongue, and hair follicles. Mutations in this gene are associated with type 1 pachyonychia congenita, non-epidermolytic palmoplantar keratoderma and unilateral palmoplantar verrucous nevus. [provided by RefSeq, Jul 2008]

Uniprot Description

K16: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K6. K16 and K17 are coexpressed only in pathological situations such as metaplasias and carcinomas of the uterine cervix and in psoriasis vulgaris.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: cytoskeleton; intermediate filament; nucleus

Molecular Function: protein binding; structural constituent of cytoskeleton

Biological Process: epidermis development; intermediate filament cytoskeleton organization and biogenesis; cytoskeleton organization and biogenesis; keratinocyte migration; hair cycle; keratinocyte differentiation; cell proliferation; keratinization; morphogenesis of an epithelium; innate immune response; inflammatory response; negative regulation of cell migration; aging

Disease: Palmoplantar Keratoderma, Nonepidermolytic, Focal; Pachyonychia Congenita 1