Mouse anti-Human, Mouse Cytokeratin 10 Monoclonal Antibody | anti-KRT10 antibody

Cytokeratin 10 (BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT10) (BSA & Azide Free) (MaxLight 490)

Cat. Num. AAA6204642

• Gene Names: KRT10; BIE; EHK; K10; KPP; BCIE; CK10
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: Cytokeratin 10; Monoclonal Antibody; Cytokeratin 10 (BCIE; BIE; EHK; Keratin Type I Cytoskeletal 10; KRT10) (BSA & Azide Free) (MaxLight 490); BCIE; KRT10; anti-KRT10 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: SPM261
• Specificity: Recognizes Cytokeratin 10. Species Crossreactivity: Human, Mouse
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-KRT10 antibody: Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: IP: 0.5-1ug/500ug protein lysate; FC/FACS: 0.5-1ug/million cells; IHC: 0.5-1.0ug/ml for 30 minutes at RT; IF: 0.5-1ug/ml; WB: 0.25-0.5ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Skin extract of a human Psoriasis patient. Cellular Localization: Cytoplasmic
• Conjugate: MaxLight490
• Positive Control: A431, HeLa, MCF7 cells or Esophagus.
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Product Categories/Family for anti-KRT10 antibody

Antibodies; Cytokeratin

References

1. Barrott JJ et al. Proc Natl Acad Sci U S A 108:12752-7 (2011) 2. Reichelt J et al. J Cell Sci 110 (Pt 18):2175-86 (1997)

NCBI and Uniprot Product Information

• NCBI GI #: 195972866
• NCBI GeneID: 3858
• NCBI Accession #: NP_000412.3
• NCBI GenBank Nucleotide #: NM_000421.3
• UniProt Accession #: P13645; Q14664; Q8N175
• Molecular Weight: 58,827 Da
• NCBI Official Full Name: keratin, type I cytoskeletal 10
• NCBI Official Synonym Full Names: keratin 10
• NCBI Official Symbol: KRT10
• NCBI Official Synonym Symbols: BIE; EHK; K10; KPP; BCIE; CK10
• NCBI Protein Information: keratin, type I cytoskeletal 10; CK-10; keratin-10; cytokeratin 10
• UniProt Protein Name: Keratin, type I cytoskeletal 10
• Protein Family: Keratin
• UniProt Gene Name: KRT10
• UniProt Synonym Gene Names: KPP; CK-10; K10
• UniProt Entry Name: K1C10_HUMAN

NCBI Description

This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21. [provided by RefSeq, Jul 2008]

Uniprot Description

K10: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K1.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: extracellular space; membrane; keratin filament; cytoplasm; intermediate filament; nucleus

Molecular Function: structural constituent of epidermis

Biological Process: keratinocyte differentiation

Disease: Erythroderma, Ichthyosiform, Congenital Reticular; Epidermolytic Hyperkeratosis; Ichthyosis, Cyclic, With Epidermolytic Hyperkeratosis