Rabbit anti-Rat Cystatin C Monoclonal Antibody | anti-CST3 antibody

Recombinant Anti-Cystatin C Antibody, Rabbit Monoclonal

Cat. Num. AAA8112682

• Gene Names: Cst3; CYSC
• Reactivity: Rat
• Applications: ELISA
• Purity: Protein A
• Synonyms: Cystatin C; Monoclonal Antibody; Recombinant Anti-Cystatin C Antibody; Rabbit Monoclonal; Cystatin C/CST3 Antibody; Rabbit MAb; cystatin C; anti-CST3 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 002
• Specificity: Rat Cystatin C/CST3.; Has cross-reactivity in ELISA with Mouse CST3, Human CST3; Has cross-reactivity in ELISA with Mouse CST3, Human CST3
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CST3 antibody: ELISA (EIA)
• Application Notes: ELISA: 1:5000-1:10,000
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Rat Cystatin C/CST3 protein
• Conjugation: Unconjugated
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Rat Cystatin C/CST3 (rR Cystatin C/CST3).
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-CST3 antibody

Cystatin C, also known as Cystatin-3 (CST3) is a secreted type 2 cysteine protease inhibitor synthesized in all nucleated cells, has been proposed as a replacement for serum creatinine for the assessment of renal function, particularly to detect small reductions in glomerular filtration rate. The mature, active form of human cystatin C is a single non-glycosylated polypeptide chain consisting of 120 amino acid residues, with a molecular mass of 13, 343-13, 359 Da, and containing four characteristic disulfide-paired cysteine residues. Cystatin C is a low-molecular-weight protein that has been proposed as a marker of renal function that could replace creatinine. Indeed, the concentration of Cystatin C is mainly determined by glomerular filtration and is particularly of interest in clinical settings where the relationship between creatinine production and muscle mass impairs the clinical performance of creatinine. Since the last decade, numerous studies have evaluated its potential use in measuring renal function in various populations. More recently, other potential developments for its clinical use have emerged. In almost all the clinical studies, Cystatin C demonstrated a better accuracy than serum creatinine in discriminating normal from impaired kidney function, but controversial results have been obtained by comparing this protein with other indices of kidney disease, especially serum creatinine-based equations, such as early atherosclerosis, Alzheimer's dementia, vascular aneurysms, hyperhomocysteinaemia and other neurodegenerative diseases. Cystatin C could be a useful clinical tool to identify HIV-infected persons. In addition, its expression is up-regulated in malignance of certain tumor progression.

References

Mares J, et al. (2003) Use of cystatin C determination in clinical diagnostics. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 147(2): 177-80.Mussap M, et al. (2004) Biochemistry and clinical role of human cystatin C. Crit Rev Clin Lab Sci. 41(5-6): 467-550.Sronie-Vivien S, et al. (2008) Cystatin C: current position and future prospects. Clin Chem Lab Med. 46(12): 1664-86.Taglieri N, et al. (2009) Cystatin C and cardiovascular risk. Clin Chem. 55(11): 1932-43.

NCBI and Uniprot Product Information

• NCBI GI #: 54234046
• NCBI GeneID: 25307
• NCBI Accession #: NP_036969.1
• NCBI GenBank Nucleotide #: NM_012837.1
• UniProt Accession #: P14841; Q5M968
• Molecular Weight: 15,437 Da
• NCBI Official Full Name: cystatin-C
• NCBI Official Synonym Full Names: cystatin C
• NCBI Official Symbol: Cst3
• NCBI Official Synonym Symbols: CYSC
• NCBI Protein Information: cystatin-C; Cystatin C (cysteine proteinase inhibitor); cystatin 3; cystatin-3
• UniProt Protein Name: Cystatin-C
• Protein Family: Cystatin
• UniProt Gene Name: Cst3
• UniProt Entry Name: CYTC_RAT

NCBI Description

extracellular cysteine protease inhibitor that may be involved in amyloidosis [RGD, Feb 2006]

Uniprot Description

CST3: As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. Defects in CST3 are the cause of amyloidosis type 6 (AMYL6); also known as hereditary cerebral hemorrhage with amyloidosis (HCHWA), cerebral amyloid angiopathy (CAA) or cerebroarterial amyloidosis Icelandic type. AMYL6 is a hereditary generalized amyloidosis due to cystatin C amyloid deposition. Cystatin C amyloid accumulates in the walls of arteries, arterioles, and sometimes capillaries and veins of the brain, and in various organs including lymphoid tissue, spleen, salivary glands, and seminal vesicles. Amyloid deposition in the cerebral vessels results in cerebral amyloid angiopathy, cerebral hemorrhage and premature stroke. Cystatin C levels in the cerebrospinal fluid are abnormally low. Genetic variations in CST3 are associated with age- related macular degeneration type 11 (ARMD11). ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane. Belongs to the cystatin family.

Protein type: Secreted, signal peptide; Inhibitor; Secreted

Cellular Component: extracellular space; nuclear membrane; contractile fiber; lysosome; endoplasmic reticulum; extracellular region; multivesicular body; cell projection; cell soma; perinuclear region of cytoplasm; axon; cytoplasm; basement membrane; vesicle

Molecular Function: protease binding; protease inhibitor activity; beta-amyloid binding; endopeptidase inhibitor activity; cysteine protease inhibitor activity

Biological Process: salivary gland development; apoptosis; response to toxin; negative regulation of peptidase activity; defense response; response to organic cyclic substance; response to carbohydrate stimulus; response to estradiol stimulus; positive regulation of cell proliferation; fibril organization and biogenesis; circadian sleep/wake cycle, REM sleep; response to axon injury; negative regulation of proteolysis; response to drug; response to nutrient levels; response to inorganic substance; male gonad development; regulation of programmed cell death; Sertoli cell development; cell activation; eye development; response to hypoxia; brain development; response to oxidative stress; positive regulation of DNA replication; embryo implantation; regulation of tissue remodeling