Immunofluorescence (IF)
(Immunofluorescence of monoclonal antibody to CYLD on HeLa cell. [antibody concentration 10ug/ml].)
Mouse anti-Human CYLD Monoclonal Antibody | anti-CYLD antibody
CYLD (Ubiquitin Carboxyl-terminal Hydrolase CYLD, Deubiquitinating Enzyme CYLD, Ubiquitin Thiolesterase CYLD, Ubiquitin-specific-processing Protease CYLD, CYLD1, KIAA0849, HSPC057) (HRP)
Gene Names
CYLD; EAC; MFT; SBS; TEM; BRSS; CDMT; MFT1; CYLD1; CYLDI; USPL2
Reactivity
Human
Applications
ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
CYLD; Monoclonal Antibody; CYLD (Ubiquitin Carboxyl-terminal Hydrolase CYLD; Deubiquitinating Enzyme CYLD; Ubiquitin Thiolesterase CYLD; Ubiquitin-specific-processing Protease CYLD; CYLD1; KIAA0849; HSPC057) (HRP); anti-CYLD antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
2C3
Specificity
Recognizes human CYLD.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Sequence Length
3540
Applicable Applications for anti-CYLD antibody
ELISA (EIA)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Partial recombinant corresponding to aa854-953 from human CYLD (AAH12342) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
QNMELFAVLCIETSHYVAFVKYGKDDSAWLFFDSMADRDGGQNGFNIPQVTPCPEVGEYLKMSLEDLHSLDSRRIQGCARRLLCDAYMCMYQSPTMSLYK
Conjugate
HRP
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunofluorescence (IF)
(Immunofluorescence of monoclonal antibody to CYLD on HeLa cell. [antibody concentration 10ug/ml].)
Immunofluorescence (IF)
(Immunofluorescence of monoclonal antibody to CYLD on HeLa cell. [antibody concentration 10ug/ml].)
Testing Data
(Detection limit for recombinant GST tagged CYLD is 0.1ng/ml as a capture antibody.)
Testing Data
(Detection limit for recombinant GST tagged CYLD is 0.1ng/ml as a capture antibody.)
Related Product Information for anti-CYLD antibody
CYLD is a 956aa, cytoplasmic, deubiquitinating enzyme belonging to the ubiquitin carboxy-terminal hydrolases (UCH) family of proteins with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains, a proline rich region, a SH3 binding domain and a sequence homology to catalytic domain of UCH. CYLD is identified as a tumor suppressor protein affecting the JNK signaling pathway. CYLD is a negative regulator of TRAF2 and NF-kappa-B signaling pathway and is also known to have receptor-dependent role in regulating the I-kappa-B kinase pathway. It also has a deubiquitinating activity that is directed towards non-Lys-48-linked polyubiquitin chains and TRAP1 is a novel substrate for deubiquitination. Mutated CYLD is known to be associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome.
Product Categories/Family for anti-CYLD antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Homo sapiens cylindromatosis (turban tumor syndrome), mRNA
NCBI Official Synonym Full Names
CYLD lysine 63 deubiquitinase
NCBI Official Symbol
CYLD
NCBI Official Synonym Symbols
EAC; MFT; SBS; TEM; BRSS; CDMT; MFT1; CYLD1; CYLDI; USPL2
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase CYLD
Protein Family
NCBI Description
This gene is encodes a cytoplasmic protein with three cytoskeletal-associated protein-glycine-conserved (CAP-GLY) domains that functions as a deubiquitinating enzyme. Mutations in this gene have been associated with cylindromatosis, multiple familial trichoepithelioma, and Brooke-Spiegler syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Research Articles on CYLD
Similar Products
Product Notes
The CYLD (Catalog #AAA6152044) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CYLD (Ubiquitin Carboxyl-terminal Hydrolase CYLD, Deubiquitinating Enzyme CYLD, Ubiquitin Thiolesterase CYLD, Ubiquitin-specific-processing Protease CYLD, CYLD1, KIAA0849, HSPC057) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CYLD can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CYLD for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CYLD, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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