Cyclin H Recombinant Protein | CCNH recombinant protein

Cyclin H, 1- 323aa, Human, His tag, E Coli

Cat. Num. AAA203429

• Gene Names: CCNH; CAK; p34; p37; CycH
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: Cyclin H; 1- 323aa; Human; His tag; E Coli; CCNH; CAK; p34; p37; 6330408H09Rik; AI661354; AV102684; AW538719; CDK activating kinase; Cyclin dependent kinase activating kinase; CyclinH; MO15 associated protein; P36; Cyclin-H; CCNH recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 2mM DTT, 30% glycerol, 2mM EDTA, 0.1M NaCl
• Concentration: 1mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MYHNSSQKRH WTFSSEEQLA RLRADANRKF RCKAVANGKV LPNDPVFLEP HEEMTLCKYY EKRLLEFCSV FKPAMPRSVV GTACMYFKRF YLNNSVMEYH PRIIMLTCAF LACKVDEFNV SSPQFVGNLR ESPLGQEKAL EQILEYELLL IQQLNFHLIV HNPYRPFEGF LIDLKTRYPI LENPEILRKT ADDFLNRIAL TDAYLLYTPS QIALTAILSS ASRAGITMES YLSESLMLKE NRTCLSQLLD IMKSMRNLVK KYEPPRSEEV AVLKQKLERC HSAELALNVI TKKRKGYEDD DYVSKKSKHE EEEWTDDDLV ESL
• Sequence Length: 323

• Applicable Applications for CCNH recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for CCNH recombinant protein

Cyclin H, also known as CDK7, is a member of the cyclin-dependent protein kinase family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin H forms a trimeric complex with cyclin H and MAT1, which functions as a CDK-activating kinase (CAK). Cyclin H is an essential component of the transcription factor TFIIH that is involved in transcription initiation and DNA repair. Recombinant human Cyclin H, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for CCNH recombinant protein

Cell Cycle

References

Schneider, E., et al. (1998) Oncogene. 17 (21):2733-41.; Yee A., et al. (1995) Canser Res. 55 (24):6058-62.;

NCBI and Uniprot Product Information

• NCBI GI #: 4502623
• NCBI GeneID: 902
• NCBI Accession #: NP_001230
• NCBI GenBank Nucleotide #: NM_001239.3
• UniProt Accession #: P51946; Q53X72; Q8TBL9
• Molecular Weight: 39.8 kDa (343aa), confirmed by MALDI-TOF
• NCBI Official Full Name: cyclin-H isoform 1
• NCBI Official Synonym Full Names: cyclin H
• NCBI Official Symbol: CCNH
• NCBI Official Synonym Symbols: CAK; p34; p37; CycH
• NCBI Protein Information: cyclin-H
• UniProt Protein Name: Cyclin-H
• Protein Family: Cyclin
• UniProt Gene Name: CCNH

NCBI Description

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK). This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery. A pseudogene of this gene is found on chromosome 4. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Nov 2010]

Uniprot Description

Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.