Mouse Cyclin A Monoclonal Antibody | anti-CCNA antibody
Cyclin A (CCN1, CCNA, CCNA2, Cyclin A2) (BSA & Azi168744-AFde Free) (MaxLight 750)
Reactivity
Bovine, Human, Monkey, Mouse
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified by Protein A/G Affinity Chromatography.
Synonyms
Cyclin A; Monoclonal Antibody; Cyclin A (CCN1; CCNA; CCNA2; Cyclin A2) (BSA & Azi168744-AFde Free) (MaxLight 750); CCN1; Cyclin A2; anti-CCNA antibody
Host
Mouse
Reactivity
Bovine, Human, Monkey, Mouse
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
E67
Specificity
Recognizes bovine Cyclin A at ~54kD. Its epitope is located amino acids 144-148 of human Cyclin A2. Species Crossreactivity: mouse, human, african green monkey and mink.
Purity/Purification
Purified by Protein A/G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.
Applicable Applications for anti-CCNA antibody
Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC) Frozen/Paraffin, Immunoprecipitation (IP), Western Blot (WB)
Application Notes
IP: 1-2ug per 500ug protein
IF: 1-2ug/ml
FLISA: Suitable for coating
WB: 0.5-1ug/ml
FC/FACS: 0.5-1ug per million cells
IHC-F/P: 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Applications are based on unconjugated antibody.
IF: 1-2ug/ml
FLISA: Suitable for coating
WB: 0.5-1ug/ml
FC/FACS: 0.5-1ug per million cells
IHC-F/P: 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Applications are based on unconjugated antibody.
Immunogen
Full length bovine Cyclin A protein. Cellular Localization: Predominantly nuclear
Conjugate
MaxLight750
Positive Control
K-562 and HeLa Cells
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Product Categories/Family for anti-CCNA antibody
References
1. Adamczewski, J.P., Gannon, J.V. & Hunt, T., Simian virus 40 large T antigen associates with cyclin A and p33cdk2. Journal of Virology, 67(11): 6551-6557 (1993).
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Product Notes
The CCNA (Catalog #AAA6236450) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Cyclin A (CCN1, CCNA, CCNA2, Cyclin A2) (BSA & Azi168744-AFde Free) (MaxLight 750) reacts with Bovine, Human, Monkey, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Cyclin A can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC) Frozen/Paraffin, Immunoprecipitation (IP), Western Blot (WB). IP: 1-2ug per 500ug protein IF: 1-2ug/ml FLISA: Suitable for coating WB: 0.5-1ug/ml FC/FACS: 0.5-1ug per million cells IHC-F/P: 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CCNA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cyclin A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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