Western Blot (WB)
(Western Blot detection against Immunogen (37.11kD).)
Mouse anti-Human CYB5R4 Monoclonal Antibody | anti-CYB5R4 antibody
CYB5R4 (Cytochrome b5 Reductase 4, Flavohemoprotein b5/b5R, b5+b5R, N-terminal Cytochrome B5 and Cytochrome B5 Oxidoreductase Domain-containing Protein, cb5/cb5R, NCB5OR) (AP)
Gene Names
CYB5R4; NCB5OR; cb5/cb5R; dJ676J13.1
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
CYB5R4; Monoclonal Antibody; CYB5R4 (Cytochrome b5 Reductase 4; Flavohemoprotein b5/b5R; b5+b5R; N-terminal Cytochrome B5 and Cytochrome B5 Oxidoreductase Domain-containing Protein; cb5/cb5R; NCB5OR) (AP); anti-CYB5R4 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
1E8
Specificity
Recognizes human CYB5R4.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-CYB5R4 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Partial recombinant corresponding to aa388-488 from human CYB5R4 (NP_057314) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
VKLMFFNKTEDDIIWRSQLEKLAFKDKRLDVEFVLSAPISEWNGKQGHISPALLSEFLKRNLDKSKVLVCICGPVPFTEQGVRLLHDLNFSKNEIHSFTA
Conjugate
AP
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western Blot detection against Immunogen (37.11kD).)
Western Blot (WB)
(Western Blot detection against Immunogen (37.11kD).)
Western Blot (WB)
(CYB5R4 monoclonal antibody, Western Blot analysis of CYB5R4 expression in HeLa.)
Western Blot (WB)
(CYB5R4 monoclonal antibody, Western Blot analysis of CYB5R4 expression in HeLa.)
Testing Data
(Detection limit for recombinant GST tagged CYB5R4 is ~0.03ng/ml as a capture antibody.)
Testing Data
(Detection limit for recombinant GST tagged CYB5R4 is ~0.03ng/ml as a capture antibody.)
Related Product Information for anti-CYB5R4 antibody
NADH-cytochrome b5 reductase involved in endoplasmic reticulum stress response pathway. Plays a critical role in protecting pancreatic beta-cells against oxidant stress, possibly by protecting the cell from excess buildup of reactive oxygen species (ROS). Reduces a variety of substrates in vitro, such as cytochrome c, feericyanide and methemoglobin.
Product Categories/Family for anti-CYB5R4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
59,474 Da
NCBI Official Full Name
cytochrome b5 reductase 4
NCBI Official Synonym Full Names
cytochrome b5 reductase 4
NCBI Official Symbol
CYB5R4
NCBI Official Synonym Symbols
NCB5OR; cb5/cb5R; dJ676J13.1
NCBI Protein Information
cytochrome b5 reductase 4; N-terminal cytochrome b5 and cytochrome b5 oxidoreductase domain-containing protein; NADPH cytochrome B5 oxidoreductase; cytochrome b-type NAD(P)H oxidoreductase; flavohemoprotein b5+b5R; flavohemoprotein b5/b5R
UniProt Protein Name
Cytochrome b5 reductase 4
Protein Family
UniProt Gene Name
CYB5R4
UniProt Synonym Gene Names
NCB5OR; b5+b5R
UniProt Entry Name
NB5R4_HUMAN
Similar Products
Product Notes
The CYB5R4 cyb5r4 (Catalog #AAA6130823) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CYB5R4 (Cytochrome b5 Reductase 4, Flavohemoprotein b5/b5R, b5+b5R, N-terminal Cytochrome B5 and Cytochrome B5 Oxidoreductase Domain-containing Protein, cb5/cb5R, NCB5OR) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CYB5R4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the CYB5R4 cyb5r4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CYB5R4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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