Mouse anti-Human CUX1 / CASP Monoclonal Antibody | anti-CUX1 antibody

Mouse Monoclonal [clone 2A10] (IgG1,k) to Human CUX1 / CASP

Cat. Num. AAA246172

• Gene Names: CUX1; CDP; CUX; p75; CASP; CDP1; COY1; Clox; p100; p110; p200; CUTL1; GOLIM6; CDP/Cut; Cux/CDP; Nbla10317
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: CUX1 / CASP; Monoclonal Antibody; Mouse Monoclonal [clone 2A10] (IgG1; k) to Human CUX1 / CASP; Anti-CUX1 / CASP Antibody (clone 2A10) IHC-plus; CUX1; CASP; CDP1; Clox; COY1; CUTL1; CCAAT displacement protein; Cut homolog; CUX; CUT; GOLIM6; Homeobox protein cux-1; p100; p200; p75; Protein CASP; CDP; Cut-like homeobox 1; Nbla10317; p110; Human CUX1; anti-CUX1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 2A10
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2. Sourced in Ascites.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 678

• Applicable Applications for anti-CUX1 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), WB; Usage: Immunofluorescence (10 ug/ml) - positive control HeLa cells. Sandwich ELISA (Recombinant protein).
• Target Species: Human
• Immunogen Description: CUTL1 (AAH25422, 521 a.a. ~ 621 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen Type: Recombinant protein
• Immunogen: CUX1 / CASP antibody was raised against cUTL1 (AAH25422, 521 a.a. ~ 621 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Anti-CUX1 / CUTL1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-CUX1 / CUTL1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to CUTL1 on HeLa cell (antibody concentration 10 ug/ml).)

Western Blot (WB)

(Western blot of CUX1 expression in transfected 293T cell line by CUTL1 monoclonal antibody, clone 2A10. Lane 1: Transfected cell lysate Lane 2: Non-transfected lysate.)

ELISA (EIA)

(Detection limit for recombinant GST tagged CUTL1 is approximately 0.03 ng/ml as a capture antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 31652236
• NCBI GeneID: 1523
• NCBI Accession #: NP_001904.2
• NCBI GenBank Nucleotide #: NM_001913.4
• UniProt Accession #: Q13948; Q53GU9; Q8TBS3; B3KWH3; B3KWH8; B4DZZ2; G3V1Z6; J3KPQ6; P39880
• Molecular Weight: 75,644 Da
• NCBI Official Full Name: protein CASP isoform b
• NCBI Official Synonym Full Names: cut-like homeobox 1
• NCBI Official Symbol: CUX1
• NCBI Official Synonym Symbols: CDP; CUX; p75; CASP; CDP1; COY1; Clox; p100; p110; p200; CUTL1; GOLIM6; CDP/Cut; Cux/CDP; Nbla10317
• NCBI Protein Information: protein CASP; cut homolog; homeobox protein cux-1; CCAAT displacement protein; golgi integral membrane protein 6; putative protein product of Nbla10317
• UniProt Protein Name: Protein CASP
• Protein Family: Homeobox protein
• UniProt Gene Name: CUX1
• UniProt Synonym Gene Names: CUTL1
• UniProt Entry Name: CASP_HUMAN

NCBI Description

The protein encoded by this gene is a member of the homeodomain family of DNA binding proteins. It may regulate gene expression, morphogenesis, and differentiation and it may also play a role in the cell cycle progession. Several alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Feb 2011]

Uniprot Description

CUTL1 iso1: Probably has a broad role in mammalian development as a repressor of developmentally regulated gene expression. May act by preventing binding of positively-activing CCAAT factors to promoters. Component of nf-munr repressor; binds to the matrix attachment regions (MARs) (5' and 3') of the immunoglobulin heavy chain enhancer. Represses T-cell receptor (TCR) beta enhancer function by binding to MARbeta, an ATC-rich DNA sequence located upstream of the TCR beta enhancer. Belongs to the CUT homeobox family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: integral to Golgi membrane; cytosol; nucleus

Molecular Function: sequence-specific DNA binding; chromatin binding

Biological Process: regulation of transcription from RNA polymerase II promoter; intra-Golgi vesicle-mediated transport; transcription, DNA-dependent; multicellular organismal development; positive regulation of transcription from RNA polymerase II promoter; auditory receptor cell differentiation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of dendrite morphogenesis; kidney development; lung development