Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Prolow-density lipoprotein receptor-related protein 1 (LRP1) Standard Curve.)
Mouse Prolow-Density Lipoprotein Receptor-Related Protein 1 (LRP1) ELISA Kit | LRP1 elisa kit
Mouse Prolow-Density Lipoprotein Receptor-Related Protein 1 (LRP1) ELISA Kit
Gene Names
Lrp1; Lrp; A2mr; CD91; AI316852; b2b1554Clo
Reactivity
Mouse
Synonyms
Prolow-Density Lipoprotein Receptor-Related Protein 1 (LRP1); Mouse Prolow-Density Lipoprotein Receptor-Related Protein 1 (LRP1) ELISA Kit; LRP1; A2MR; APOER; APR; CD91; FLJ16451; IGFBP3R; LRP; MGC88725; TGFBR5; TbetaR-V/LRP-1/IGFBP-3 receptor; alpha-2-macroglobulin receptor; low density lipoprotein-related protein 1; type V tgf-beta receptor; LRP1 elisa kit
Reactivity
Mouse
Specificity
Mouse Prolow-density lipoprotein receptor-related protein 1 (LRP1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse LRP1. No significant cross-reactivity or interference between Mouse LRP1 and analogues was observed.
Sequence Length
4545
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Prolow-density lipoprotein receptor-related protein 1 (LRP1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Prolow-density lipoprotein receptor-related protein 1 (LRP1) Standard Curve.)
Related Product Information for LRP1 elisa kit
Description: This Mouse Prolow-density lipoprotein receptor-related protein 1 (LRP1) ELISA Kit employs a two-site sandwich ELISA to quantitate LRP1 in samples. An antibody specific for LRP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LRP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LRP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LRP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Low density lipoprotein receptor-related protein 1 (alpha-2-macroglobulin receptor), also known as LRP1 or CD91, is a protein forming a receptor found in the plasma membrane of human cells involved in receptor-mediated endocytosis.Neurons require cholesterol to function. Cholesterol is imported into the neuron by the apolipoprotein apoE4 via LRP1 receptors on the cell surface. It is thought that a causal factor in Alzheimer's is the malfunction of this process which damages neurons by starving them of cholesterol.The protein showed strong calcium binding. Kristensen et al. (1990) and Strickland et al. (1990) demonstrated that LRP is identical to the alpha-2-macroglobulin (A2M) receptor (A2MR). Like the mannose-6-phosphate receptor, the A2MR/LRP molecule is probably bifunctional.
Background: Low density lipoprotein receptor-related protein 1 (alpha-2-macroglobulin receptor), also known as LRP1 or CD91, is a protein forming a receptor found in the plasma membrane of human cells involved in receptor-mediated endocytosis.Neurons require cholesterol to function. Cholesterol is imported into the neuron by the apolipoprotein apoE4 via LRP1 receptors on the cell surface. It is thought that a causal factor in Alzheimer's is the malfunction of this process which damages neurons by starving them of cholesterol.The protein showed strong calcium binding. Kristensen et al. (1990) and Strickland et al. (1990) demonstrated that LRP is identical to the alpha-2-macroglobulin (A2M) receptor (A2MR). Like the mannose-6-phosphate receptor, the A2MR/LRP molecule is probably bifunctional.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
prolow-density lipoprotein receptor-related protein 1
NCBI Official Synonym Full Names
low density lipoprotein receptor-related protein 1
NCBI Official Symbol
Lrp1
NCBI Official Synonym Symbols
Lrp; A2mr; CD91; AI316852; b2b1554Clo
NCBI Protein Information
prolow-density lipoprotein receptor-related protein 1
UniProt Protein Name
Prolow-density lipoprotein receptor-related protein 1
Protein Family
UniProt Gene Name
Lrp1
UniProt Synonym Gene Names
LRP-1; A2MR; LRP-85; LRP-515; LRPICD
Uniprot Description
Endocytic receptor involved in endocytosis and in phagocytosis of apoptotic cells. Required for early embryonic development. Involved in cellular lipid homeostasis. Involved in the plasma clearance of chylomicron remnants and activated LRPAP1 (alpha 2-macroglobulin), as well as the local metabolism of complexes between plasminogen activators and their endogenous inhibitors. May modulate cellular events, such as APP metabolism, kinase-dependent intracellular signaling, neuronal calcium signaling as well as neurotransmission.
Research Articles on LRP1
Similar Products
Product Notes
The Mouse LRP1 lrp1 (Catalog #AAA9717768) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717768 ELISA Kit recognizes Mouse LRP1. It is sometimes possible for the material contained within the vial of "Prolow-Density Lipoprotein Receptor-Related Protein 1 (LRP1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
