Mouse CSK Monoclonal Antibody | anti-CSK antibody

CSK antibody

Cat. Num. AAA5310908

• Applications: ELISA, Immunofluorescence, Western Blot
• Synonyms: CSK; Monoclonal Antibody; CSK antibody; Mouse monoclonal CSK antibody; Monoclonal CSK antibody; Anti-CSK antibody; C-Src Tyrosine Kinase antibody; Carboxy-terminal Src kinase antibody; MGC117393 antibody; anti-CSK antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 5F3
• Form/Format: Liquid in ascitic fluid containing 0.03% sodium azide.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 450

• Applicable Applications for anti-CSK antibody: ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: ELISA: 1:10,000; IF: 1:200-1:1000; WB: 1:500-1:2000
• Immunogen: CSK antibody was raised in Mouse using a purified recombinant fragment of human CSK expressed in E Coli as the immunogen.
• Cross-Reactivity: Human, Mouse, Monkey, Rat
• Preparation and Storage: Store at 4 degree C for short term storage.; Store at-20 degree C for long term storage.; Avoid repeated freeze/thaw cycles.

Flow Cytometry (FC/FACS)

(FC analysis of HL-60 cells using CSK antibody (green) and negative control (purple).)

Western Blot (WB)

(Western Blot showing CSK antibody used against NIH/3T3 (1), Hela (2), COS7 (3), Jurkat (4), Raw246.7 (5), A549 (6), HL-60 (7) and PC-12 (8) cell lysate.)

Immunofluorescence (IF)

(Immunofluorescence analysis of U251 cells using CSK antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)

Related Product Information for anti-CSK antibody

CSK is a ubiquitously expressed nonreceptor tyrosine kinase that negatively regulates the Src family kinases (SFK) by phosphorylation of the SFK carboxy-terminal tyrosine. Phosphorylated carboxy-terminal tyrosine binds to the SH2 domain of SFK intramolecularly and leads to folding and inactivation of the SFK. This Csk-catalyzed SFK tyrosine phosphorylation is highly specific and exclusive. The SFK carboxy-terminal tyrosine is the only known physiological substrate of Csk.

Product Categories/Family for anti-CSK antibody

Proteases; Inhibitors; & Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 49456875
• NCBI GeneID: 1445
• UniProt Accession #: P41240; Q2M3N2; Q6FGZ6
• Molecular Weight: 50,704 Da
• NCBI Official Full Name: CSK, partial
• NCBI Official Synonym Full Names: C-terminal Src kinase
• NCBI Official Symbol: CSK
• NCBI Protein Information: tyrosine-protein kinase CSK
• UniProt Protein Name: Tyrosine-protein kinase CSK
• Protein Family: Chloroplast sensor kinase
• UniProt Gene Name: CSK

NCBI Description

The protein encoded by this gene is involved in multiple pathways, including the regulation of Src family kinases. It plays an important role in T-cell activation through its association with the protein encoded by the protein tyrosine phosphatase, non-receptor type 22 (PTPN22) gene. This protein also phosphorylates C-terminal tyrosine residues on multiple substrates, including the protein encoded by the SRC proto-oncogene, non-receptor tyrosine kinase gene. Phosphorylation suppresses the kinase activity of the Src family tyrosine kinases. An intronic polymorphism (rs34933034) in this gene has been found to affect B-cell activation and is associated with systemic lupus erythematosus (SLE). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2017]

Uniprot Description

Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T-cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK.