Mouse Crystallin Alpha B Monoclonal Antibody | anti-CRYAB antibody
Mouse Anti Human Crystallin Alpha B
Applications
ELISA, Western Blot
Purity
CRYAB antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Synonyms
Crystallin Alpha B; Monoclonal Antibody; Mouse Anti Human Crystallin Alpha B; CRYAB Antibody; Mouse Anti Human; CRYA2; CTPP2; HSPB5; CRYAB; anti-CRYAB antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b heavy chains and kappa light chain
Clone Number
P2E8AT
Purity/Purification
CRYAB antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Form/Format
1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
Sterile Filtered colorless solution.
Sterile Filtered colorless solution.
Sequence Length
175
Applicable Applications for anti-CRYAB antibody
ELISA (EIA), Western Blot (WB)
Application Notes
CRYAB antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 2,000. Recommended starting dilution is 1:1,000.
Immunogen
Anti-human CRYAB mAb, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with recombinant human CRYAB amino acids 1-175 purified from E Coli.
Preparation and Storage
For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles.
12 months at -20 degree C. 1 month at 4 degree C.
12 months at -20 degree C. 1 month at 4 degree C.
Related Product Information for anti-CRYAB antibody
Introduction: Alpha crystallins are composed of two gene products; alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20). They act as molecular chaperones and hold them in in large soluble aggregates. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional function of ï¡-crystallins are an autokinase activity and participation in the intracellular architecture. Alpha-B is expressed widely in many tissues and organs and occurs in many neurological diseases.
Product Categories/Family for anti-CRYAB antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20,129 Da
NCBI Official Symbol
CRYAB
NCBI Protein Information
crystallin, alpha B; Alpha(B)-crystallin; Alpha-crystallin B chain
UniProt Protein Name
Alpha-crystallin B chain
Protein Family
UniProt Gene Name
CRYAB
UniProt Entry Name
CRYAB_PIG
Uniprot Description
CRYAB: a major structural protein of the eye lens. A member of the small heat shock protein (sHSP also known as the HSP20) family. Alpha-B is expressed in the lens as well as other tissues. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy.
Protein type: Chaperone; Heat shock protein
Cellular Component: mitochondrion; cytoplasm; plasma membrane; nucleus; Z disc; cytosol
Molecular Function: protein homodimerization activity; metal ion binding; unfolded protein binding; structural constituent of eye lens
Biological Process: lens development in camera-type eye; tubulin folding; muscle development; negative regulation of intracellular transport; response to hydrogen peroxide; response to hypoxia; negative regulation of caspase activity; protein homooligomerization
Similar Products
Product Notes
The CRYAB cryab (Catalog #AAA140203) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Crystallin Alpha B can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). CRYAB antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 2,000. Recommended starting dilution is 1:1,000. Researchers should empirically determine the suitability of the CRYAB cryab for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Crystallin Alpha B, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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