Mouse CRYM Monoclonal Antibody | anti-CRYM antibody

CRYM antibody

Cat. Num. AAA831031

• Gene Names: CRYM; THBP; DFNA40
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: CRYM antibody was purified by affinity chromatography.
• Synonyms: CRYM; Monoclonal Antibody; CRYM antibody; Monoclonal CRYM; Anti-CRYM; Crystallin Mu; DFNA40; THBP; anti-CRYM antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 1B5
• Purity/Purification: CRYM antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 500 ug-1 mg/ml (varies by lot)
• Sequence Length: 314

• Applicable Applications for anti-CRYM antibody: Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IF: 1:100; IHC: 1:300; WB: 1:2000
• Biological Significance: Crystallins are separated into two classes: taxon-specific and ubiquitous. The former class is also called phylogenetically-restricted crystallins. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. This gene encodes a taxon-specific crystallin protein that binds NADPH and has sequence similarity to bacterial ornithine cyclodeamin ases.
• Cross-Reactivity: Human
• Immunogen: CRYM antibody was raised in mouse using a full length recombinant protein of human CRYM (NP_001879) produced in HEK293T cells, as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant CRYM protein (Right) or empty vector (Left) detected with CRYM antibody)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of CRYM protein in paraffin embedded Human prostate tissue using CRYM antibody)

Related Product Information for anti-CRYM antibody

Mouse monoclonal CRYM antibody

Product Categories/Family for anti-CRYM antibody

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 4503065
• NCBI GeneID: 1428
• NCBI Accession #: NP_001879
• NCBI GenBank Nucleotide #: NM_001888.3
• UniProt Accession #: Q14894; Q5HYB7; D5MNX0
• Molecular Weight: 33,776 Da
• NCBI Official Full Name: ketimine reductase mu-crystallin
• NCBI Official Synonym Full Names: crystallin, mu
• NCBI Official Symbol: CRYM
• NCBI Official Synonym Symbols: THBP; DFNA40
• NCBI Protein Information: ketimine reductase mu-crystallin
• UniProt Protein Name: Ketimine reductase mu-crystallin
• Protein Family: Ketimine reductase
• UniProt Gene Name: CRYM
• UniProt Synonym Gene Names: THBP
• UniProt Entry Name: CRYM_HUMAN

NCBI Description

Crystallins are separated into two classes: taxon-specific and ubiquitous. The former class is also called phylogenetically-restricted crystallins. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. This gene encodes a taxon-specific crystallin protein that binds NADPH and has sequence similarity to bacterial ornithine cyclodeaminases. The encoded protein does not perform a structural role in lens tissue, and instead it binds thyroid hormone for possible regulatory or developmental roles. Mutations in this gene have been associated with autosomal dominant non-syndromic deafness. [provided by RefSeq, Sep 2014]

Uniprot Description

CRYM: Specifically catalyzes the reduction of imine bonds in brain substrates that may include cystathionine ketimine (CysK) and lanthionine ketimine (LK). Binds thyroid hormone which is a strong reversible inhibitor. Presumably involved in the regulation of the free intracellular concentration of triiodothyronine and access to its nuclear receptors. Belongs to the ornithine cyclodeaminase family.

Protein type: EC 1.5.1.25; Lyase

Chromosomal Location of Human Ortholog: 16p12.2

Cellular Component: mitochondrion; cytoplasm; nucleus

Molecular Function: thiomorpholine-carboxylate dehydrogenase activity; protein homodimerization activity; NADP binding; transcription corepressor activity

Biological Process: sensory perception of sound; negative regulation of transcription from RNA polymerase II promoter; thyroid hormone metabolic process

Disease: Deafness, Autosomal Dominant 40