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Western Blot (WB)
(Western Blot detection against Immunogen (70.18kD).)
Mouse anti-Human CPB1 Monoclonal Antibody | anti-CPB1 antibody
CPB1 (Carboxypeptidase B, Pancreas-specific Protein, PASP, CPB, PCPB)
Reactivity
Human
Applications
ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
CPB1; Monoclonal Antibody; CPB1 (Carboxypeptidase B; Pancreas-specific Protein; PASP; CPB; PCPB); Anti -CPB1 (Carboxypeptidase B; anti-CPB1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
4D5
Specificity
Recognizes human CPB1.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Sequence
SAHHGGEHFEGEKVFRVNVEDENHINIIRELASTTQIDFWKPDSVTQIKPHSTVDFRVKAEDTVTVENVLKQNELQYKVLISNLRNVVEAQFDSRVRATGHSYEKYNKWETIEAWTQQVATENPALISRSVIGTTFEGRAIYLLKVGKAGQNKPAIFMDCGFHAREWISPAFCQWFVREAVRTYGREIQVTELLDKLDFYVLPVLNIDGYIYTWTKSRFWRKTRSTHTGSSCIGTDPNRNFDAGWCEIGASRNPCDETYCGPAAESEKETKALADFIRNKLSSIKAYLTIHSYSQMMIYPYSYAYKLGENNAELNALAKATVKELASLHGTKYTYGPGATTIYPAAGGSDDWAYDQGIRYSFTFELRDTGRYGFLLPESQIRATCEETFLAIKYVASYVLEHLY
Applicable Applications for anti-CPB1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry.
Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml
Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml
Immunogen
Full length recombinant corresponding to aa14-417 from human CPB1 (AAH15338) with GST tag. MW of the GST tag alone is 26kD.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot detection against Immunogen (70.18kD).)
Western Blot (WB)
(Western Blot detection against Immunogen (70.18kD).)
Western Blot (WB)
(Western Blot analysis of CPB1 expression in transfected 293T cell line by CPB1 monoclonal antibody.|Lane 1: CPB1 transfected lysate (47.4kD).|Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of CPB1 expression in transfected 293T cell line by CPB1 monoclonal antibody.|Lane 1: CPB1 transfected lysate (47.4kD).|Lane 2: Non-transfected lysate.)
Immunohistochemistry (IHC)
(Immunoperoxidase of monoclonal antibody to CPB1 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3ug/ml].)
Immunohistochemistry (IHC)
(Immunoperoxidase of monoclonal antibody to CPB1 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3ug/ml].)
Immunoprecipitation (IP)
(Immunoprecipitation of CPB1 transfected lysate using CPB1 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with CPB1 rabbit polyclonal antibody.)
Immunoprecipitation (IP)
(Immunoprecipitation of CPB1 transfected lysate using CPB1 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with CPB1 rabbit polyclonal antibody.)
Related Product Information for anti-CPB1 antibody
Carboxypeptidases are enzymes that hydrolyze C-terminal peptide bonds. The carboxypeptidase family includes metallo-, serine, and cysteine carboxypeptidases. According to their substrate specificity, these enzymes are referred to as carboxypeptidase A (cleaving aliphatic residues) or carboxypeptidase B (cleaving basic amino residues). CPB2 is activated by trypsin and acts on carboxypeptidase B substrates. After thrombin activation, the mature protein downregulates fibrinolysis. Polymorphisms have been described for its gene and its promoter region.
Product Categories/Family for anti-CPB1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
carboxypeptidase B preproprotein
NCBI Official Synonym Full Names
carboxypeptidase B1 (tissue)
NCBI Official Symbol
CPB1
NCBI Protein Information
carboxypeptidase B
Similar Products
Product Notes
The CPB1 (Catalog #AAA6002187) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CPB1 (Carboxypeptidase B, Pancreas-specific Protein, PASP, CPB, PCPB) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CPB1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml. Researchers should empirically determine the suitability of the CPB1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SAHHGGEHFE GEKVFRVNVE DENHINIIRE LASTTQIDFW KPDSVTQIKP HSTVDFRVKA EDTVTVENVL KQNELQYKVL ISNLRNVVEA QFDSRVRATG HSYEKYNKWE TIEAWTQQVA TENPALISRS VIGTTFEGRA IYLLKVGKAG QNKPAIFMDC GFHAREWISP AFCQWFVREA VRTYGREIQV TELLDKLDFY VLPVLNIDGY IYTWTKSRFW RKTRSTHTGS SCIGTDPNRN FDAGWCEIGA SRNPCDETYC GPAAESEKET KALADFIRNK LSSIKAYLTI HSYSQMMIYP YSYAYKLGEN NAELNALAKA TVKELASLHG TKYTYGPGAT TIYPAAGGSD DWAYDQGIRY SFTFELRDTG RYGFLLPESQ IRATCEETFL AIKYVASYVL EHLY. It is sometimes possible for the material contained within the vial of "CPB1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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