Mouse anti-Human CPA1 Monoclonal Antibody | anti-CPA1 antibody

CPA1 (Carboxypeptidase A1, CPA) (AP)

Cat. Num. AAA6130723

• Gene Names: CPA1; CPA
• Reactivity: Human
• Applications: ELISA, Immunoprecipitation, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CPA1; Monoclonal Antibody; CPA1 (Carboxypeptidase A1; CPA) (AP); anti-CPA1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 3F11
• Specificity: Recognizes human CPA1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-CPA1 antibody: ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-419 from human CPA1 (AAH05279) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MRGLLVLSVLLGAVFGKEDFVGHQVLRISVADEAQVQKVKELEDLEHLQLDFWRGPAHPGSPIDVRVPFPSIQAVKIFLESHGISYETMIEDVQSLLDEEQEQMFAFRSRARSTDTFNYATYHTLEEIYDFLDLLVAENPHLVSKIQIGNTYEGRPIYVLKFSTGGSKRPAIWIDTGIHSREWVTQASGVWFAKKITQDYGQDAAFTAILDTLDIFLEIVTNPDGFAFTHSTNRMWRKTRSHTAGSLCIGVDPNRNWDAGFGLSGASSNPCSETYRGKFANSEVEVKSIVDFVKDHGNIKAFISIHSYSQLLMYPYGYKTEPVPDQDELDQLSKAAVTALASLYGTKFNYGSIIKAIYQASGSTIDWTYSQGIKYSFTFELRDTGRYGFLLPASQIIPTAKETWLALLTIMEHTLNHPY
• Conjugate: AP
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (71.83kD).)

Western Blot (WB)

(Western Blot analysis of CPA1 expression in transfected 293T cell line by CPA1 monoclonal antibody. Lane 1: CPA1 transfected lysate (47.1kD). Lane 2: Non-transfected lysate.)

Immunoprecipitation (IP)

(Immunoprecipitation of CPA1 transfected lysate using CPA1 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with CPA1 rabbit polyclonal antibody.)

Testing Data

(Detection limit for recombinant GST tagged CPA1 is ~0.3ng/ml as a capture antibody.)

Related Product Information for anti-CPA1 antibody

Three different forms of human pancreatic procarboxypeptidase A have been isolated. The A1 and A2 forms are monomeric proteins with different biochemical properties. Carboxypeptidase A1 is a monomeric pancreatic exopeptidase. It is involved in zymogen inhibition.

Product Categories/Family for anti-CPA1 antibody

Antibodies; Enzymes; Carboxypeptidase

NCBI and Uniprot Product Information

• NCBI GI #: 13528974
• NCBI GeneID: 1357
• Molecular Weight: 47,140 Da
• NCBI Official Full Name: Homo sapiens carboxypeptidase A1 (pancreatic), mRNA
• NCBI Official Synonym Full Names: carboxypeptidase A1
• NCBI Official Symbol: CPA1
• NCBI Official Synonym Symbols: CPA
• NCBI Protein Information: carboxypeptidase A1
• Protein Family: Carboxypeptidase

NCBI Description

This gene encodes a member of the carboxypeptidase A family of zinc metalloproteases. This enzyme is produced in the pancreas and preferentially cleaves C-terminal branched-chain and aromatic amino acids from dietary proteins. This gene and several family members are present in a gene cluster on chromosome 7. Mutations in this gene may be linked to chronic pancreatitis, while elevated protein levels may be associated with pancreatic cancer. [provided by RefSeq, Jan 2015]