Immunofluorescence (IF)
(Immunofluorescence staining of SARS-CoV-2 (2019-nCoV) Spike in ACE2-overexpressed 293T cells, infected (left) or non-infected (right) by 2019-nCOV-Spike pseudovirus (PSV). Cells were blocked with 10% serum, and incubated with SARS-CoV Spike monoclonal antibody (dilution ratio 1:60) at 37 degree C for 1 hour. Then cells were stained with the FITC-conjugated Goat Anti-human IgG secondary antibody (green).)
COVID 19 Spike S Coronavirus Monoclonal Antibody | anti-COVID-19 antibody
SARS-CoV/SARS-CoV-2 Spike antibody, Chimeric MAb
Applications
ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Synonyms
COVID 19 Spike S Coronavirus; Monoclonal Antibody; SARS-CoV/SARS-CoV-2 Spike antibody; Chimeric MAb; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Spike S; anti-COVID-19 antibody
Host
Human; the antibody is a chimeric monoclonal antibody with a mouse variable region and a human kappa/IgG1 constant region.
Clonality
Monoclonal
Isotype
Human IgG1 kappa
Clone Number
D004
Specificity
SARS-CoV Spike RBD Protein has cross-reactivity in ELISA with SARS-CoV Spike S1 Protein, SARS-CoV-2 (2019-nCoV) Spike S1 Protein, SARS-CoV-2 (2019-nCoV) Spike RBD Protein.
Form/Format
0.2um filtered solution in PBS
Applicable Applications for anti-COVID-19 antibody
ELISA (EIA), Immunohistochemistry -Paraffin (IHC-P), Flow Cytometry (FC/FACS/FCM), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
ELISA1:5000-1:10000
Immunogen
Recombinant SARS-CoV Spike RBD Protein
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Short term: -20°C
Long term : -80°C
Long term : -80°C
Immunofluorescence (IF)
(Immunofluorescence staining of SARS-CoV-2 (2019-nCoV) Spike in ACE2-overexpressed 293T cells, infected (left) or non-infected (right) by 2019-nCOV-Spike pseudovirus (PSV). Cells were blocked with 10% serum, and incubated with SARS-CoV Spike monoclonal antibody (dilution ratio 1:60) at 37 degree C for 1 hour. Then cells were stained with the FITC-conjugated Goat Anti-human IgG secondary antibody (green).)
Immunofluorescence (IF)
(Immunofluorescence staining of SARS-CoV-2 (2019-nCoV) Spike in ACE2-overexpressed 293T cells, infected (left) or non-infected (right) by 2019-nCOV-Spike pseudovirus (PSV). Cells were blocked with 10% serum, and incubated with SARS-CoV Spike monoclonal antibody (dilution ratio 1:60) at 37 degree C for 1 hour. Then cells were stained with the FITC-conjugated Goat Anti-human IgG secondary antibody (green).)
Related Product Information for anti-COVID-19 antibody
Preparation:
It is a chimeric monoclonal antibody combining the constant domains of the human IgG1 molecule with mouse variable regions. The variable region was obtained from a mouse immunized with purified, recombinant SARS-CoV Spike RBD Protein. The antibody was produced using recombinant antibody technology.
It is a chimeric monoclonal antibody combining the constant domains of the human IgG1 molecule with mouse variable regions. The variable region was obtained from a mouse immunized with purified, recombinant SARS-CoV Spike RBD Protein. The antibody was produced using recombinant antibody technology.
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Product Notes
The COVID-19 (Catalog #AAA8574847) is an Antibody produced from Human; the antibody is a chimeric monoclonal antibody with a mouse variable region and a human kappa/IgG1 constant region. and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's COVID 19 Spike S Coronavirus can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry -Paraffin (IHC-P), Flow Cytometry (FC/FACS/FCM), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP). ELISA1:5000-1:10000. Researchers should empirically determine the suitability of the COVID-19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COVID 19 Spike S Coronavirus, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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