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Western Blot (WB) (Western blot analysis of extracts of various cell lines, using POLD2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

Rabbit POLD2 Polyclonal Antibody | anti-POLD2 antibody

POLD2

Reactivity
Human, Mouse, Rat
Applications
Western Blot
Synonyms
POLD2; Polyclonal Antibody; anti-POLD2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Sequence Length
469
Applicable Applications for anti-POLD2 antibody
Western Blot (WB)
Species
Human
Immunogen
Recombinant Protein

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using POLD2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

Western Blot (WB) (Western blot analysis of extracts of various cell lines, using POLD2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Product Categories/Family for anti-POLD2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
469
NCBI Official Full Name
DNA polymerase delta subunit 2
NCBI Official Synonym Full Names
polymerase (DNA directed), delta 2, accessory subunit
NCBI Official Symbol
POLD2
NCBI Protein Information
DNA polymerase delta subunit 2; Pol delta B subunit (p50); DNA polymerase delta subunit p50; polymerase (DNA directed), delta 2, regulatory subunit 50kDa
UniProt Protein Name
DNA polymerase delta subunit 2
Protein Family
UniProt Gene Name
POLD2
UniProt Entry Name
DPOD2_HUMAN

NCBI Description

This gene encodes the 50-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. The encoded protein is required for the stimulation of DNA polymerase delta activity by the processivity cofactor proliferating cell nuclear antigen (PCNA). Expression of this gene may be a marker for ovarian carcinomas. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Mar 2012]

Uniprot Description

POLD2: The function of the small subunit is not yet clear. Belongs to the DNA polymerase delta/II small subunit family.

Protein type: DNA repair, damage; Nucleotide Metabolism - pyrimidine; Transferase; Nucleotide Metabolism - purine; EC 2.7.7.7; DNA replication

Chromosomal Location of Human Ortholog: 7p13

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; DNA binding; DNA-directed DNA polymerase activity

Biological Process: telomere maintenance via semi-conservative replication; mismatch repair; base-excision repair; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; nucleotide-excision repair, DNA gap filling; DNA strand elongation during DNA replication; mitotic cell cycle; DNA replication; DNA repair; telomere maintenance

Research Articles on POLD2

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Product Notes

The POLD2 pold2 (Catalog #AAA9126999) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The POLD2 reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's POLD2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the POLD2 pold2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POLD2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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