Western Blot (WB)
(Figure-1: Western Blot analysis of SARS-CoV-2 Nucleocapsid Antibody: Anti- SARS-CoV-2 Nucleocapsid Antibody (Clone: ABM1F11.1E1) was used at 2ug/ml on (1) SARS-CoV-2 virus infected Vero Cell lysate and (2) Mock infected lysate.)
Mouse COVID 19 Nucleocapsid (NP) Coronavirus Monoclonal Antibody | anti-COVID-19 antibody
Monoclonal Antibody to SARS-CoV-2 Nucleocapsid (Clone: ABM1F11.1E1)
Applications
ELISA, Western Blot
Synonyms
COVID 19 Nucleocapsid (NP) Coronavirus; Monoclonal Antibody; Monoclonal Antibody to SARS-CoV-2 Nucleocapsid (Clone: ABM1F11.1E1); 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Nucleocapsid; Nucleoprotein (NP); anti-COVID-19 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
Mouse IgG1, kappa
Clone Number
ABM1F11.1E1
Form/Format
25ug in 50ul/100ug in 200ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-COVID-19 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
WB: 0.1-1ug/ml
ELISA: 1ug/ml
ELISA: 1ug/ml
Immunogen
Full length recombinant SARS-CoV-2 nucleocapsid Protein was used as the immunogen for this antibody.
Preparation and Storage
Store the antibody at 4 degree C, stable for 6 months. For long-term storage, store at -20 degree C. Avoid repeated freeze and thaw cycles.
Western Blot (WB)
(Figure-1: Western Blot analysis of SARS-CoV-2 Nucleocapsid Antibody: Anti- SARS-CoV-2 Nucleocapsid Antibody (Clone: ABM1F11.1E1) was used at 2ug/ml on (1) SARS-CoV-2 virus infected Vero Cell lysate and (2) Mock infected lysate.)
Western Blot (WB)
(Figure-1: Western Blot analysis of SARS-CoV-2 Nucleocapsid Antibody: Anti- SARS-CoV-2 Nucleocapsid Antibody (Clone: ABM1F11.1E1) was used at 2ug/ml on (1) SARS-CoV-2 virus infected Vero Cell lysate and (2) Mock infected lysate.)
Western Blot (WB)
(Figure-2: Western Blot analysis: The purified antibodies 1F11.1E1 was tested on Nucleocapsid Recombinant protein at different concentrations, 0.1 (lane 1), 0.5 (lane 2), and 1.0ug/ml (lane 3), (4) RBD protein, (5)unrelated protein 1, (6) unrelated protein 2, (7) unrelated protein 3, to detect the specific binding. 25 ng of proteins was loaded per lane.)
Western Blot (WB)
(Figure-2: Western Blot analysis: The purified antibodies 1F11.1E1 was tested on Nucleocapsid Recombinant protein at different concentrations, 0.1 (lane 1), 0.5 (lane 2), and 1.0ug/ml (lane 3), (4) RBD protein, (5)unrelated protein 1, (6) unrelated protein 2, (7) unrelated protein 3, to detect the specific binding. 25 ng of proteins was loaded per lane.)
ELISA (EIA)
(Figure-3: An indirect ELISA is carried out by coating nucleocapsid protein in serial dilution from 20 ng to 0.312 ng and using 100 ng of purified monoclonal antibodies 1F11.1E1. Peroxidase conjugated Goat-Anti mouse antibody was used at 1:5000 dilution.)
ELISA (EIA)
(Figure-3: An indirect ELISA is carried out by coating nucleocapsid protein in serial dilution from 20 ng to 0.312 ng and using 100 ng of purified monoclonal antibodies 1F11.1E1. Peroxidase conjugated Goat-Anti mouse antibody was used at 1:5000 dilution.)
Related Product Information for anti-COVID-19 antibody
The structural nucleocapsid (N) protein of nCoV/SARS-CoV-2/COVID-19 is a predicted 46 kDa phosphoprotein having 419 amino acid residues. A short Serine rich stretch and a recognized nuclear localization signal are the unique features of the nucleocapsid protein of nCoV/SARS-CoV-2/COVID-19, which seems to have a little homology with the proteins of other members of this large corona virus family. These features also suggest the involvement of nucleocapsid protein of nCoV/SARS-CoV-2/COVID-19 in different stages of viral lifecycle. The protein has multifaceted activities including packing of the nCoV/SARS-CoV-2/COVID-19 viral genome into a helical ribonucleocapsid (RNP) and playing an important role in viral self-assembly causing nCoV/SARS-CoV-2/COVID-19. The nucleocapsid protein of nCoV/SARS-CoV-2/COVID-19 also forms dimer in the cell by self-association with the help of interactive C terminal domain.
Product Categories/Family for anti-COVID-19 antibody
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Product Notes
The COVID-19 (Catalog #AAA669304) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's COVID 19 Nucleocapsid (NP) Coronavirus can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). WB: 0.1-1ug/ml ELISA: 1ug/ml. Researchers should empirically determine the suitability of the COVID-19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COVID 19 Nucleocapsid (NP) Coronavirus, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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