Mouse anti-Human CORTICOTROPHIN RELEASING FACTOR Monoclonal Antibody | anti-CRH antibody
MOUSE ANTI HUMAN CORTICOTROPHIN RELEASING FACTOR
Gene Names
CRH; CRF
Reactivity
Human
Applications
Immunohistochemistry, Immunofluorescence, ELISA, Immunohistochemistry
Synonyms
CORTICOTROPHIN RELEASING FACTOR; Monoclonal Antibody; MOUSE ANTI HUMAN CORTICOTROPHIN RELEASING FACTOR; anti-CRH antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
4H9
Specificity
CORTICOTROPHIN RELEASING FACTOR
This item is specific for corticotrophin releasing factor (CRF), a hormone found mainly in the hypothalamus that regulates the release of corticotrophin from the pituitary gland.
The unique lysine residue (Lys-36) is likely to be involved in the antigenic determinant as lysine modification by CA blocks binding completely. Human serum, containing CRF-binding protein, also inhibits the binding of this antibody to CRF.
This item is specific for corticotrophin releasing factor (CRF), a hormone found mainly in the hypothalamus that regulates the release of corticotrophin from the pituitary gland.
The unique lysine residue (Lys-36) is likely to be involved in the antigenic determinant as lysine modification by CA blocks binding completely. Human serum, containing CRF-binding protein, also inhibits the binding of this antibody to CRF.
Form/Format
Purified (Purified Ig - liquid)
Concentration
1mg/ml (varies by lot)
Sequence Length
196
Applicable Applications for anti-CRH antibody
Immunohistology - Frozen (IHC), Immunofluorescence (IF), ELISA (EIA), Immunohistology - Paraffin (IHC)
Application Notes
Immunohistology - Paraffin: Min Dilution: 1/100
Max Dilution: 1/7000
Immunohistology: *This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Sodium citrate buffer pH 6.0 is recommended for this purpose. Enzyme digestion with 0.1% trypsin may also be necessary.
Max Dilution: 1/7000
Immunohistology: *This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Sodium citrate buffer pH 6.0 is recommended for this purpose. Enzyme digestion with 0.1% trypsin may also be necessary.
Immunogen
Corticotrophin Releasing Factor conjugated to Bovine Serum Albumin
Affinity
Averages 10(e)-9 M with native and modified CRF.
Preparation
Purified Ig prepared by affinity chromatography on Protein G
Preservative Stabilizers
0.09% Sodium Azide (NaN3)
Buffer Solution
Phosphate buffered saline
Preparation and Storage
Store at +4 degree C or at -20 degree C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of dispatch.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of dispatch.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,422 Da
NCBI Official Full Name
corticoliberin preproprotein
NCBI Official Synonym Full Names
corticotropin releasing hormone
NCBI Official Symbol
CRH
NCBI Official Synonym Symbols
CRF
NCBI Protein Information
corticoliberin; corticotropin-releasing factor
UniProt Protein Name
Corticoliberin
Protein Family
UniProt Gene Name
CRH
UniProt Synonym Gene Names
CRF
UniProt Entry Name
CRF_HUMAN
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Product Notes
The CRH crh (Catalog #AAA211554) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI HUMAN CORTICOTROPHIN RELEASING FACTOR reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CORTICOTROPHIN RELEASING FACTOR can be used in a range of immunoassay formats including, but not limited to, Immunohistology - Frozen (IHC), Immunofluorescence (IF), ELISA (EIA), Immunohistology - Paraffin (IHC). Immunohistology - Paraffin: Min Dilution: 1/100 Max Dilution: 1/7000 Immunohistology: *This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Enzyme digestion with 0.1% trypsin may also be necessary. Researchers should empirically determine the suitability of the CRH crh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CORTICOTROPHIN RELEASING FACTOR, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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