Mouse anti-Human, Mouse COPS5 / JAB1 Monoclonal Antibody | anti-COPS5 antibody

Mouse Monoclonal [clone 2A10.8] (IgG2b) to Mouse COPS5 / JAB1

Cat. Num. AAA245067

• Gene Names: COPS5; CSN5; JAB1; SGN5; MOV-34
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, Immunoprecipitation
• Purity: Protein G Purified
• Synonyms: COPS5 / JAB1; Monoclonal Antibody; Mouse Monoclonal [clone 2A10.8] (IgG2b) to Mouse COPS5 / JAB1; Anti-COPS5 / JAB1 Antibody (clone 2A10.8) IHC-plus; COPS5; 38 kDa Mov34 homolog; JAB1; Signalosome subunit 5; MOV-34; SGN5; CSN5; Mouse COPS5; anti-COPS5 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 2A10.8
• Specificity: The full-length mouse JAB-1 gene expressed in E Coli.
• Purity/Purification: Protein G Purified
• Form/Format: PBS, pH 7.4. Sourced in Ascites.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 334

• Applicable Applications for anti-COPS5 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: IF, IHC-P (5 ug/ml), IP, WB; Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Mouse
• Immunogen Description: Recombinant mouse COPS5.
• Immunogen Type: Recombinant protein
• Immunogen: COPS5 / JAB1 antibody was raised against recombinant mouse COPS5.
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-COPS5 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 38027923
• NCBI GeneID: 10987
• NCBI Accession #: NP_006828.2
• NCBI GenBank Nucleotide #: NM_006837.2
• UniProt Accession #: Q92905; O15386; Q6AW95; Q86WQ4; Q9BQ17
• Molecular Weight: 37,579 Da
• NCBI Official Full Name: COP9 signalosome complex subunit 5
• NCBI Official Synonym Full Names: COP9 signalosome subunit 5
• NCBI Official Symbol: COPS5
• NCBI Official Synonym Symbols: CSN5; JAB1; SGN5; MOV-34
• NCBI Protein Information: COP9 signalosome complex subunit 5; 38 kDa Mov34 homolog; signalosome subunit 5; jun activation domain-binding protein 1; COP9 constitutive photomorphogenic homolog subunit 5
• UniProt Protein Name: COP9 signalosome complex subunit 5
• UniProt Gene Name: COPS5
• UniProt Synonym Gene Names: CSN5; JAB1; SGN5; Signalosome subunit 5
• UniProt Entry Name: CSN5_HUMAN

NCBI Description

The protein encoded by this gene is one of the eight subunits of COP9 signalosome, a highly conserved protein complex that functions as an important regulator in multiple signaling pathways. The structure and function of COP9 signalosome is similar to that of the 19S regulatory particle of 26S proteasome. COP9 signalosome has been shown to interact with SCF-type E3 ubiquitin ligases and act as a positive regulator of E3 ubiquitin ligases. This protein is reported to be involved in the degradation of cyclin-dependent kinase inhibitor CDKN1B/p27Kip1. It is also known to be an coactivator that increases the specificity of JUN/AP1 transcription factors. [provided by RefSeq, Jul 2008]

Uniprot Description

COPS5: Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex. Interacts directly with a large number of proteins that are regulated by the CSN complex, confirming a key role in the complex. Component of the CSN complex, composed of COPS1/GPS1, COPS2, COPS3, COPS4, COPS5, COP6, COPS7 (COPS7A or COPS7B) and COPS8. In the complex, it probably interacts directly with COPS1, COPS2, COPS4, COPS6 and COPS7 (COPS7A or COPS7B). The CSN complex interacts with the BRISC complex. Also exists as monomeric form. Interacts with TP53, MIF, JUN, UCHL1, NCOA1, HIF1A, p27Kip1, BCL3, GFER, PGR, LHCGR, SMAD4, SMAD7, ID1, ID3, ITGB2 and TOP2A. Part of a complex consisting of RANBP9, Ran, DYRK1B and COPS5. Belongs to the peptidase M67A family. CSN5 subfamily.

Protein type: EC 3.4.-.-; Protease; Translation; Nuclear receptor co-regulator; Adaptor/scaffold; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 8q13.1

Cellular Component: nucleoplasm; signalosome; eukaryotic translation initiation factor 3 complex; synaptic vesicle; perinuclear region of cytoplasm; cytoplasm; cell junction; nucleus

Molecular Function: protein binding; metallopeptidase activity; metal ion binding; translation initiation factor activity; ubiquitin-specific protease activity; transcription coactivator activity

Biological Process: transcription from RNA polymerase II promoter; protein deneddylation; cullin deneddylation; protein deubiquitination; translation; regulation of cell cycle; translational initiation; positive regulation of transcription from RNA polymerase II promoter; proteolysis; regulation of JNK cascade