Mouse anti-Human Complement Factor H-Related Protein 5 Monoclonal Antibody | anti-CFHR5 antibody

Complement Factor H-Related Protein 5 (FHR5)

Cat. Num. AAA600911

• Gene Names: CFHR5; FHR5; CFHL5; FHR-5; FLJ10549; MGC133240
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Complement Factor H-Related Protein 5; Monoclonal Antibody; Complement Factor H-Related Protein 5 (FHR5); Anti -Complement Factor H-Related Protein 5 (FHR5); anti-CFHR5 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 7H187
• Specificity: Recognizes human Complement Factor H-Related 5.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.

• Applicable Applications for anti-CFHR5 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Western Blot, Immunoprecipitation and Direct ELISA.; Dilution: Western Blot: 1ug/ml. C7850-88A under non-reducing conditions only.; Immunoprecipitation: 25ug/ml. Conditioned cell culture medium spiked with C7850-88A
• Immunogen: Recombinant protein corresponding to aa19-569 of human Complement Factor H-Related 5 expressed in NSO cells (Accession #Q9BXR6)
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-CFHR5 antibody

Complement factor H­related protein 5 (FHR­5 or CFHR5) is a 65 kDa member of the factor H­related protein family. It is secreted by the liver and circulates either free or bound to HDL. It is unique among FHR proteins in that it associates with complexes that arise from complement activation. It promotes C3b cleavage and blocks C3 convertase activity. Human CFHR5 is 551 amino acids (aa) in length. It contains nine, 60 aa SUSHI­, or short consensus repeats, plus 18 intrachain disulfide bonds. SUSHI repeats 5­7 (aa 267­444) reportedly mediate CFHR5 binding to CRP and heparin. The complement interaction site(s) is unknown. Human CFHR5 shares 64% aa sequence identity with canine CFHR5..

Product Categories/Family for anti-CFHR5 antibody

Antibodies; Abs to Complement

NCBI and Uniprot Product Information

• NCBI GI #: 13540563
• NCBI GeneID: 81494
• NCBI Accession #: NP_110414.1
• NCBI GenBank Nucleotide #: NM_030787.3
• UniProt Accession #: Q9BXR6; Q2NKK2; Q05CF5; Q5VYL6
• Molecular Weight: 64,419 Da
• NCBI Official Full Name: complement factor H-related protein 5
• NCBI Official Synonym Full Names: complement factor H-related 5
• NCBI Official Symbol: CFHR5
• NCBI Official Synonym Symbols: FHR5; CFHL5; FHR-5; FLJ10549; MGC133240
• NCBI Protein Information: complement factor H-related protein 5; OTTHUMP00000034672
• UniProt Protein Name: Complement factor H-related protein 5
• Protein Family: Complement factor H-related protein
• UniProt Gene Name: CFHR5
• UniProt Synonym Gene Names: CFHL5; FHR5
• UniProt Entry Name: FHR5_HUMAN

NCBI Description

This gene is a member of a small complement factor H (CFH) gene cluster on chromosome 1. Each member of this gene family contains multiple short consensus repeats (SCRs) typical of regulators of complement activation. The protein encoded by this gene has nine SCRs with the first two repeats having heparin binding properties, a region within repeats 5-7 having heparin binding and C reactive protein binding properties, and the C-terminal repeats being similar to a complement component 3 b (C3b) binding domain. This protein co-localizes with C3, binds C3b in a dose-dependent manner, and is recruited to tissues damaged by C-reactive protein. Allelic variations in this gene have been associated, but not causally linked, with two different forms of kidney disease: membranoproliferative glomerulonephritis type II (MPGNII) and hemolytic uraemic syndrome (HUS). [provided by RefSeq]

Uniprot Description

CFHR5: Involved in complement regulation. Defects in CFHR5 have been found in patients with atypical hemolytic uremic syndrome and may contribute to the disease. Atypical hemolytic uremic syndrome is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q31.3

Cellular Component: extracellular region

Biological Process: complement activation, alternative pathway

Disease: Cfhr5 Deficiency