Mouse anti-Human Complement 4d (C4d) Monoclonal Antibody | anti-C4d antibody

Complement 4d (C4d) (Acute Humoral Rejection Marker)

Cat. Num. AAA4381316

• Reactivity: Human
• Applications: ELISA, Immunofluorescence, Immunohistochemistry
• Purity: Purified Ab conjugated to CF488
• Synonyms: Complement 4d (C4d); Monoclonal Antibody; Complement 4d (C4d) (Acute Humoral Rejection Marker); Acidic Complement C4; Basic C4; C4-1; C4a Anaphylatoxin; C4A; C4A2; C4A3; C4A4; C4A6; C4b; C4B1; C4B12; C4d fragment; C4F; C4S; Complement C4 Gamma Chain; Complement Component 4A; Complement Component 4B; anti-C4d antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: C4D204
• Specificity: This MAb is specific to Complement 4d (C4d) and reacts with the secreted as well as cell-bound C4d.C4d is a degradation product of the activated complement factor C4b. Complement 4b is typically activated by binding of Abs to specific target molecules. Following activation and degradation of the C4 molecule, thio-ester groups are exposed, which allow transient, covalent binding of the degradation product Complement 4d to endothelial cell surfaces and extracellular matrix components of vascular basement membranes near the sites of C4 activation. The presence of C4d in peritubular capillaries is a key indicator for acute humoral (i.e. antibody-mediated) rejection of kidney, heart, pancreas and lung allografts. As an established marker of antibody-mediated acute renal allograft rejection and its proclivity for endothelium, this component can be detected in peritubular capillaries in chronic renal allograft rejection as well as hyperacute rejection, acute vascular rejection, acute cellular rejection, and borderline rejection. It has been shown to be a significant predictor of transplant kidney graft survival. Anti-C4d, combined with anti-C3d, can be utilized as a tool for allograft rejection that may warrant a prompt and aggressive anti-rejection treatment.
• Purity/Purification: Purified Ab conjugated to CF488
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

• Applicable Applications for anti-C4d antibody: ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin
• Application Notes: ELISA (For coating use Ab at 1-2ug/ml order Ab without BSA); IF: 1:50-1:100; Immunohistochemistry (Formalin-fixed) (1:200-1:400 for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes)
• Positive Control: Rejected Renal Transplant Tissue.
• Immunogen: Recombinant human Complement 4d protein
• Cellular Localization: Intracytoplasmic vacuoles of endothelial cells and Secreted
• Hu-chromosome Location: 6p21.3
• Preparation and Storage: Antibody with azide: store at 4 to 8 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-Page

(SDS-PAGE Analysis Purified Complement 4d Mouse Monoclonal Antibody (C4D204). Confirmation of Integrity and Purity of Antibody.)

References

1. Sacks SH et. al. Curr Opin Nephrol Hypertens. 2002;11(6):627-8. 2. Collins AB et. al. J Am Soc Nephrol. 1999;10(10):2208-14. 3. Racusen LC et. al. Am J Transplant. 2003;3(6):708-14.