Mouse anti-Human COMP/THBS5 Monoclonal Antibody | anti-COMP/THBS5 antibody

Mouse anti Human COMP/THBS5 Monoclonal Antibody

Cat. Num. AAA2543269

• Gene Names: COMP; MED; EDM1; EPD1; TSP5; PSACH; THBS5
• Reactivity: Human
• Applications: ELISA
• Synonyms: COMP/THBS5; Monoclonal Antibody; Mouse anti Human COMP/THBS5 Monoclonal Antibody; anti-COMP/THBS5 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Sequence Length: 757

• Applicable Applications for anti-COMP/THBS5 antibody: ELISA (EIA)
• Application Notes: ELISA: 0.5-1 mug/mL. This antibody can be used at 0.5-1 mug/mL with the appropriate secondary reagents to detect COMP. The detection limit for COMP is approximately 0.078 ng/well.
• Immunogen: Recombinant Human COMP protein
• Buffer: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human COMP (rhCOMP; NP_000086.2; Met 1-Ala 757). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2 degree C- 8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -70 degree C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

NCBI and Uniprot Product Information

• NCBI GI #: 40217843
• NCBI GeneID: 1311
• NCBI GenBank Nucleotide #: NM_000095.2
• Molecular Weight: 77,214 Da
• NCBI Official Full Name: cartilage oligomeric matrix protein
• NCBI Official Synonym Full Names: cartilage oligomeric matrix protein
• NCBI Official Symbol: COMP
• NCBI Official Synonym Symbols: MED; EDM1; EPD1; TSP5; PSACH; THBS5
• NCBI Protein Information: cartilage oligomeric matrix protein
• UniProt Protein Name: Cartilage oligomeric matrix protein
• UniProt Gene Name: COMP
• UniProt Synonym Gene Names: COMP; TSP5
• UniProt Entry Name: COMP_HUMAN

NCBI Description

The protein encoded by this gene is a noncollagenous extracellular matrix (ECM) protein. It consists of five identical glycoprotein subunits, each with EGF-like and calcium-binding (thrombospondin-like) domains. Oligomerization results from formation of a five-stranded coiled coil and disulfides. Binding to other ECM proteins such as collagen appears to depend on divalent cations. Contraction or expansion of a 5 aa aspartate repeat and other mutations can cause pseudochondroplasia (PSACH) and multiple epiphyseal dysplasia (MED). [provided by RefSeq, Jul 2016]

Uniprot Description

COMP: May play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Could play a role in the pathogenesis of osteoarthritis. Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7. Defects in COMP are the cause of multiple epiphyseal dysplasia type 1 (EDM1). EDM is a generalized skeletal dysplasia associated with significant morbidity. Joint pain, joint deformity, waddling gait, and short stature are the main clinical signs and symptoms. EDM is broadly categorized into the more severe Fairbank and the milder Ribbing types. Defects in COMP are the cause of pseudoachondroplasia (PSACH). PSAC is a dominantly inherited chondrodysplasia characterized by short stature and early-onset osteoarthrosis. PSACH is more severe than EDM1 and is recognized in early childhood. Belongs to the thrombospondin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: extracellular matrix; extracellular region; extracellular space

Molecular Function: calcium ion binding; collagen binding; extracellular matrix structural constituent; heparan sulfate proteoglycan binding; heparin binding; protease binding; protein binding

Biological Process: extracellular matrix organization and biogenesis; limb development; negative regulation of apoptosis; organ morphogenesis; skeletal development

Disease: Epiphyseal Dysplasia, Multiple, 1; Pseudoachondroplasia