Rabbit anti-Human CLU  Monoclonal Antibody | anti-CLU  antibody

Rabbit anti Human CLU  Monoclonal Antibody

Cat. Num. AAA2544225

• Gene Names: CLU; CLI; AAG4; APOJ; CLU1; CLU2; KUB1; SGP2; APO-J; SGP-2; SP-40; TRPM2; TRPM-2; NA1/NA2
• Reactivity: Human
• Applications: ELISA
• Synonyms: CLU ; Monoclonal Antibody; Rabbit anti Human CLU Monoclonal Antibody; anti-CLU antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Sequence Length: 449

• Applicable Applications for anti-CLU antibody: ELISA (EIA)
• Application Notes: ELISA: 0.1-0.2 mug/mL. This antibody can be used at 0.1-0.2 mug/mL with the appropriate secondary reagents to detect Human CLU. The detection limit for Human CLU is approximately 0.0049ng/well.
• Immunogen: Recombinant Human Clusterin/Apolipoprotein J/Apo-J protein
• Buffer: 0.2 mum filtered solution in PBS with 5% trehalose
• Preparation and Storage: This antibody can be stored at 2 degree C- 8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-CLU  antibody

Clusterin, also known as complement-associated protein SP-40, Complement cytolysis inhibitor, Apolipoprotein J, Testosterone-repressed prostate message 2, Aging-associated gene 4 protein, CLU and APOJ, is a secreted protein which belongs to the clusterin family. Clusterin/Apolipoprotein J/Apo-J is an enigmatic glycoprotein with a nearly ubiquitous tissue distribution and an apparent involvement in biological processes ranging from mammary gland involution to neurodegeneration in Alzheimer's disease. Its major form, a heterodimer, is secreted and present in physiological fluids, but truncated forms targeted to the nucleus have also been identified. Clusterin/Apolipoprotein J/Apo-J is a widely distributed glycoprotein with a wide range of biologic properties. A prominent and defining feature of clusterin is its marked induction in such disease states as glomerulonephritis, cystic renal disease, renal tubular injury, neurodegenerative conditions, atherosclerosis, and myocardial infarction. Upregulation of clusterin mRNA and protein levels detected in diverse disease states and in in vitro systems have led to suggestions that it functions in membrane lipid recycling, in apoptotic cell death, and as a stress-induced secreted chaperone protein, amongst others.

NCBI and Uniprot Product Information

• NCBI GI #: 823669086
• NCBI GeneID: 1191
• Molecular Weight: 53,643 Da
• NCBI Official Full Name: CLU, partial
• NCBI Official Synonym Full Names: clusterin
• NCBI Official Symbol: CLU
• NCBI Official Synonym Symbols: CLI; AAG4; APOJ; CLU1; CLU2; KUB1; SGP2; APO-J; SGP-2; SP-40; TRPM2; TRPM-2; NA1/NA2
• NCBI Protein Information: clusterin
• UniProt Protein Name: Clusterin
• UniProt Gene Name: CLU
• UniProt Synonym Gene Names: APOJ; CLI; KUB1; Apo-J; CLI; TRPM-2
• UniProt Entry Name: CLUS_HUMAN

NCBI Description

The protein encoded by this gene is a secreted chaperone that can under some stress conditions also be found in the cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. Alternate splicing results in both coding and non-coding variants.[provided by RefSeq, May 2011]

Uniprot Description

CLU: Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress- induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation. Belongs to the clusterin family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Mitochondrial; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8p21-p12

Cellular Component: cell surface; cytoplasm; extracellular matrix; extracellular region; extracellular space; Golgi apparatus; mitochondrion; perinuclear region of cytoplasm; protein complex

Molecular Function: ATPase activity; chaperone binding; misfolded protein binding; protein binding; ubiquitin protein ligase binding

Biological Process: activation of NF-kappaB transcription factor; cell morphogenesis; chaperone-mediated protein complex assembly; complement activation; microglial cell activation; myelin maintenance in the central nervous system; negative regulation of protein homooligomerization; platelet degranulation; positive regulation of nitric oxide biosynthetic process; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of tumor necrosis factor production; protein import; protein stabilization; release of cytochrome c from mitochondria; response to misfolded protein; response to virus; reverse cholesterol transport