Western Blot (WB)
(Western blot analysis of Cleaved PARP1 expression in Jurkat (A) whole cell lysates.)
Mouse anti-Human Cleaved PARP1 Monoclonal Antibody | anti-PARP1 antibody
Anti-Cleaved PARP1 Antibody
Western Blot (WB)
(Western blot analysis of Cleaved PARP1 expression in Jurkat (A) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of Cleaved PARP1 expression in Jurkat (A) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of Cleaved PARP1 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of Cleaved PARP1 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]
Uniprot Description
PARP1: Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP- ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LRIG3. Homo- and heterodimer with PARP2. Interacts with PARP3, APTX and SRY. The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70. Interacts with TIAM2 and ZNF423. Interacts (when poly-ADP- ribosylated) with CHD1L. Interacts with the DNA polymerase alpha catalytic subunit POLA1; this interaction functions as part of the control of replication fork progression. Interacts with EEF1A1, RNF4 and TXK.
Protein type: Nuclear envelope; EC 2.4.2.30; DNA repair, damage; Nuclear receptor co-regulator; Nucleolus; Transferase
Chromosomal Location of Human Ortholog: 1q41-q42
Cellular Component: membrane; mitochondrion; nuclear chromosome, telomeric region; nuclear envelope; nucleolus; nucleoplasm; nucleus; protein complex; transcription factor complex
Molecular Function: chromatin binding; DNA binding; DNA ligase (ATP) activity; enzyme binding; histone deacetylase binding; identical protein binding; NAD binding; NAD+ ADP-ribosyltransferase activity; protein binding; protein kinase binding; protein N-terminus binding; transcription factor binding; zinc ion binding
Biological Process: base-excision repair; behavioral response to cocaine; cellular protein metabolic process; cellular response to insulin stimulus; DNA damage response, detection of DNA damage; DNA ligation during DNA repair; DNA repair; double-strand break repair; double-strand break repair via homologous recombination; gene expression; lagging strand elongation; macrophage differentiation; mitochondrial DNA metabolic process; mitochondrial DNA repair; mitochondrion organization and biogenesis; negative regulation of transcription from RNA polymerase II promoter; nucleotide-excision repair; nucleotide-excision repair, DNA damage recognition; nucleotide-excision repair, DNA incision; positive regulation of transcription from RNA polymerase II promoter; post-translational protein modification; protein amino acid ADP-ribosylation; protein autoprocessing; protein sumoylation; regulation of growth rate; response to DNA damage stimulus; telomere maintenance; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway
Research Articles on PARP1
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Product Notes
The PARP1 parp1 (Catalog #AAA8241585) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Cleaved PARP1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cleaved PARP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: (1/2000 - 1/5000); Immunohistochemistry: (1/200 - 1/500). Researchers should empirically determine the suitability of the PARP1 parp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cleaved PARP1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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