Western Blot (WB)
(Western blot analysis of Claudin-5 was performed by loading 20ug of Mouse Placenta (lane1) and Mouse Small Intestine (lane2) lysates using Novex® NuPAGE® 4-12% Bis-Tris gel, XCell SureLock™ Electrophoresis System, Novex® Sharp Pre-Stained Protein Standard, and iBlot® Dry Blotting System. Proteins were transferred to a nitrocellulose membrane and blocked with 5 % skim milk for 1 hour at RT. Claudin-5 was detected at ~23 kDa using C5838-47B at 0.5-1ug/ml in 2.5% skim milk at 4 degree C overnight on a rocking platform. Goat Anti-Mouse IgG -HRP Secondary Antibody at 1:4000 dilution was used and chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit.)
Mouse Claudin 5 Monoclonal Antibody | anti-CLDN5 antibody
Claudin 5 (CLDN5) (FITC)
Western Blot (WB)
(Western blot analysis of Claudin-5 was performed by loading 20ug of Mouse Placenta (lane1) and Mouse Small Intestine (lane2) lysates using Novex® NuPAGE® 4-12% Bis-Tris gel, XCell SureLock™ Electrophoresis System, Novex® Sharp Pre-Stained Protein Standard, and iBlot® Dry Blotting System. Proteins were transferred to a nitrocellulose membrane and blocked with 5 % skim milk for 1 hour at RT. Claudin-5 was detected at ~23 kDa using C5838-47B at 0.5-1ug/ml in 2.5% skim milk at 4 degree C overnight on a rocking platform. Goat Anti-Mouse IgG -HRP Secondary Antibody at 1:4000 dilution was used and chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit.)
Western Blot (WB)
(Western blot analysis of Claudin-5 was performed by loading 20ug of Mouse Placenta (lane1) and Mouse Small Intestine (lane2) lysates using Novex® NuPAGE® 4-12% Bis-Tris gel, XCell SureLock™ Electrophoresis System, Novex® Sharp Pre-Stained Protein Standard, and iBlot® Dry Blotting System. Proteins were transferred to a nitrocellulose membrane and blocked with 5 % skim milk for 1 hour at RT. Claudin-5 was detected at ~23 kDa using C5838-47B at 0.5-1ug/ml in 2.5% skim milk at 4 degree C overnight on a rocking platform. Goat Anti-Mouse IgG -HRP Secondary Antibody at 1:4000 dilution was used and chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of Claudin 5 showing staining in the Membrane and Cytoplasm of paraffin-embedded human kidney tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at RT, washed with ddH2O and PBS, and then probed with C5838-47B diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4 degree C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of Claudin 5 showing staining in the Membrane and Cytoplasm of paraffin-embedded human kidney tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at RT, washed with ddH2O and PBS, and then probed with C5838-47B diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4 degree C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.)
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Product Notes
The CLDN5 (Catalog #AAA6258588) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Claudin 5 (CLDN5) (FITC) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Claudin 5 can be used in a range of immunoassay formats including, but not limited to, FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). Researchers should empirically determine the suitability of the CLDN5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Claudin 5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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