Western Blot (WB)
(Western Blot analysis of Claudin-4 was performed by loading 20ug of Mouse Small Intestine (lane1) and PC-3 (lane2) lysates using Novex® NuPAGE® 4-12 % Bis-Tris gel, XCell SureLock™ Electrophoresis System, Novex® Sharp Pre-Stained Protein Standard and iBlot® Dry Blotting System. Proteins were transferred to a nitrocellulose membrane and blocked with 5% skim milk at 4 degree C overnight. Claudin-4 was detected at ~27kD using C5838-35A at 1-3ug/ml in 2.5 % skim milk for 3 hours at RT on a rocking platform. IgG (HRP) goat anti-mouse secondary antibody at 1:4000 dilution was used and chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit.)
Mouse Claudin 4 Monoclonal Antibody | anti-CLDN4 antibody
Claudin 4 (CLDN4) (FITC)
Reactivity
Canine, Human, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography
Synonyms
Claudin 4; Monoclonal Antibody; Claudin 4 (CLDN4) (FITC); anti-CLDN4 antibody
Host
Mouse
Reactivity
Canine, Human, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
6A43
Specificity
Recognizes the human ~22kD Claudin 4 protein and does not crossreact with the protein at 55kD. Species Crossreactivity: rat ileum homogenates and canine MDCK cell lysates by Western Blot and Immunofluorescence. Reactivity has also been confirmed with formalin-fixed, paraffin-embedded (FFPE) human normal colon and colon cancer tissues by Immunohistochemistry.
Purity/Purification
Purified by Protein A Affinity Chromatography
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Fluorescein isothiocyanate (FITC).
Applicable Applications for anti-CLDN4 antibody
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Immunogen
Synthetic peptide corresponding to a 22 amino acid sequence derived from the C-terminal region of human Claudin 4.
Conjugate
FITC
Preparation and Storage
Store at -20 degree C.
Western Blot (WB)
(Western Blot analysis of Claudin-4 was performed by loading 20ug of Mouse Small Intestine (lane1) and PC-3 (lane2) lysates using Novex® NuPAGE® 4-12 % Bis-Tris gel, XCell SureLock™ Electrophoresis System, Novex® Sharp Pre-Stained Protein Standard and iBlot® Dry Blotting System. Proteins were transferred to a nitrocellulose membrane and blocked with 5% skim milk at 4 degree C overnight. Claudin-4 was detected at ~27kD using C5838-35A at 1-3ug/ml in 2.5 % skim milk for 3 hours at RT on a rocking platform. IgG (HRP) goat anti-mouse secondary antibody at 1:4000 dilution was used and chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit.)
Western Blot (WB)
(Western Blot analysis of Claudin-4 was performed by loading 20ug of Mouse Small Intestine (lane1) and PC-3 (lane2) lysates using Novex® NuPAGE® 4-12 % Bis-Tris gel, XCell SureLock™ Electrophoresis System, Novex® Sharp Pre-Stained Protein Standard and iBlot® Dry Blotting System. Proteins were transferred to a nitrocellulose membrane and blocked with 5% skim milk at 4 degree C overnight. Claudin-4 was detected at ~27kD using C5838-35A at 1-3ug/ml in 2.5 % skim milk for 3 hours at RT on a rocking platform. IgG (HRP) goat anti-mouse secondary antibody at 1:4000 dilution was used and chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit.)
Testing Data
(Human Caco-2 cells incubated with C5838-35A stained with Alexa Fluor 488 goat anti mouse secondary antinbody The DNA is counter stained with blue Hoechst 33258 stain.)
Testing Data
(Human Caco-2 cells incubated with C5838-35A stained with Alexa Fluor 488 goat anti mouse secondary antinbody The DNA is counter stained with blue Hoechst 33258 stain.)
Product Categories/Family for anti-CLDN4 antibody
References
1. Saitou, M., et al., J. Cell Biol. 141: 397-408 (1998). 2. Furuse, M., et al., J. Cell Biol. 143: 391-401 (1998). 3. Tsukita, S. & Furuse, M., Genes Cells 3: 569-573 (1998). 4. Morita, K., et al., PNAS 96: 511-516 (1999). 5. Van Itallie, C.M., et al., J. Clin. Invest. 107(10): 1319-1327 (2001).
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Product Notes
The CLDN4 (Catalog #AAA6258587) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Claudin 4 (CLDN4) (FITC) reacts with Canine, Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Claudin 4 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). Researchers should empirically determine the suitability of the CLDN4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Claudin 4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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